Please use this identifier to cite or link to this item: https://ir.swu.ac.th/jspui/handle/123456789/15069
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dc.contributor.authorWatanapokasin Y.
dc.contributor.authorChuncharunee S.
dc.contributor.authorSanmund D.
dc.contributor.authorKongnium W.
dc.contributor.authorWinichagoon P.
dc.contributor.authorRodgers G.P.
dc.contributor.authorFucharoen S.
dc.date.accessioned2021-04-05T04:32:28Z-
dc.date.available2021-04-05T04:32:28Z-
dc.date.issued2005
dc.identifier.issn0301472X
dc.identifier.other2-s2.0-28844471024
dc.identifier.urihttps://ir.swu.ac.th/jspui/handle/123456789/15069-
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-28844471024&doi=10.1016%2fj.exphem.2005.09.006&partnerID=40&md5=dec308ff9b56a09b756a4c32cc18b526
dc.description.abstractObjective. Some, but not all, β-thalassemia/hemoglobin E (β-thal/HbE) patients respond to hydroxyurea treatment. It would be helpful if patient responses to hydroxyurea could be screened in vitro to identify responders and nonresponders before beginning in vivo treatment. Materials and Methods. Thirteen β-Thal/HbE patients were treated with hydroxyurea orally for 2 years at a starting dose of 5 mg/kg/day for 5 days/week with escalation to a maximum of 10 mg/kg/day. For comparison, erythroid cells obtained from peripheral blood of the same patients 1 year after they had stopped hydroxyurea treatment were treated with hydroxyurea in vitro. The γ-globin mRNA was measured by real-time reverse-transcription polymerase chain reaction, fetal hemoglobin (HbF) by high-performance liquid chromatography, Gγ- and Aγ-globin chains by Triton X-100 acid urea polyacrylamide gel electrophoresis. Results. Treatment of cells in primary culture with 30 μM hydroxyurea for 96 hours significantly increased the fractional HbF content in β-Thal/HbE patients. The Gγ: Aγ-globin mRNA was induced 0.30- to 8-fold in vitro and 0.30- to 6-fold in vivo (r2 = 0.51, p = 0.16 by paired t-test); the fractional HbF content was induced 0.50- to 19-fold in vitro and 0.30- to 12-fold in vivo (r2 = 0.61, p = 0.20) and the Gγ: Aγ-globin chain ratio was increased 0.80- to 1.40-fold in vitro and 1- to 1.20-fold in vivo (r2 = 0.62, p = 0.13). Conclusion. The correlation of in vivo and in vitro results of HbF synthesis and globin mRNA suggest that in vitro testing may predict the in vivo response. © 2005 International Society for Experimental Hematology. Published by Elsevier Inc.
dc.subjectacid
dc.subjectgamma globin
dc.subjecthemoglobin
dc.subjecthemoglobin E
dc.subjecthemoglobin F
dc.subjecthydroxyurea
dc.subjectmessenger RNA
dc.subjecttriton x 100
dc.subjecturea
dc.subjectadult
dc.subjectarticle
dc.subjectbeta thalassemia
dc.subjectclinical article
dc.subjectcontrolled study
dc.subjectcorrelation analysis
dc.subjectdose response
dc.subjectdrug withdrawal
dc.subjecterythroid cell
dc.subjectfemale
dc.subjecthemoglobin determination
dc.subjecthemoglobin synthesis
dc.subjecthigh performance liquid chromatography
dc.subjecthuman
dc.subjecthuman cell
dc.subjecthuman cell culture
dc.subjectin vitro study
dc.subjectin vivo study
dc.subjectmale
dc.subjectperipheral circulation
dc.subjectpolyacrylamide gel electrophoresis
dc.subjectpriority journal
dc.subjectprotein content
dc.subjectprotein induction
dc.subjectprotein synthesis regulation
dc.subjectquantitative analysis
dc.subjectreal time polymerase chain reaction
dc.subjectRNA analysis
dc.subjectRNA synthesis
dc.subjectAdult
dc.subjectbeta-Thalassemia
dc.subjectCells, Cultured
dc.subjectDrug Monitoring
dc.subjectErythroid Cells
dc.subjectFemale
dc.subjectFetal Hemoglobin
dc.subjectGene Expression Regulation
dc.subjectHemoglobin E
dc.subjectHemoglobinuria
dc.subjectHumans
dc.subjectHydroxyurea
dc.subjectMale
dc.subjectMiddle Aged
dc.subjectPredictive Value of Tests
dc.subjectPrognosis
dc.titleIn vivo and in vitro studies of fetal hemoglobin induction by hydroxyurea in β-thalassemia/hemoglobin E patients
dc.typeArticle
dc.rights.holderScopus
dc.identifier.bibliograpycitationExperimental Hematology. Vol 33, No.12 (2005), p.1486-1492
dc.identifier.doi10.1016/j.exphem.2005.09.006
Appears in Collections:Scopus 1983-2021

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