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dc.contributor.authorMekseepralard C.
dc.contributor.authorToms G.L.
dc.contributor.authorRoutledge E.G.
dc.date.accessioned2021-04-05T04:32:22Z-
dc.date.available2021-04-05T04:32:22Z-
dc.date.issued2006
dc.identifier.issn221317
dc.identifier.other2-s2.0-33646146936
dc.identifier.urihttps://ir.swu.ac.th/jspui/handle/123456789/15029-
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-33646146936&doi=10.1099%2fvir.0.81660-0&partnerID=40&md5=e04892f06d8b0b390a488aa978284088
dc.description.abstractMonoclonal antibodies (mAbs) to conserved epitopes on the G glycoprotein of human respiratory syncytial virus (HRSV) subgroup A fail to neutralize the virus in cell culture in the absence of complement, but are protective in rodent models of infection. They may have potential as prophylactic agents in human infants. In order to investigate the role of Fc-dependent pathways in protection by one such antibody, 1 C2, the VH and VL genes were isolated by RT-PCR and assembled with human κ light-chain and human γ1 heavy-chain constant-region genes to form two mouse-human chimaeras, which were expressed in NSO cells. One of the chimaeras carried a wild-type γ1 chain, whilst the other had an aglycosyl mutation in the CH2 domain rendering the antibody defective in complement activation and FcγR binding. Whilst both chimaeric antibodies exhibited similar avidity for HRSV in ELISA, only the fully glycosylated wild type was capable of neutralizing the virus in the presence of complement. In mice passively immunized with either murine or wild-type γ1 chimaeric antibody, no virus could be recovered from the lungs 4 days after intranasal inoculation of HRSV. In mice immunized with the aglycosyl γ1 chimaera, however, virus was present in the lungs following challenge, although virus titres were significantly reduced compared with controls (P<0.005). These results indicate that the protective effect of this antibody is mediated by both Fc-dependent and Fc-independent pathway. © 2006 SGM.
dc.subjectalemtuzumab
dc.subjectepitope
dc.subjectFc receptor
dc.subjectimmunoglobulin Fc fragment
dc.subjectimmunoglobulin G
dc.subjectimmunoglobulin heavy chain
dc.subjectimmunoglobulin light chain
dc.subjectimmunoglobulin subclass
dc.subjectrespiratory syncytial virus vaccine
dc.subjectthrombospondin
dc.subjectanimal cell
dc.subjectanimal experiment
dc.subjectanimal model
dc.subjectanimal tissue
dc.subjectantigen binding
dc.subjectarticle
dc.subjectchimera
dc.subjectcomplement activation
dc.subjectcontrolled study
dc.subjectenzyme linked immunosorbent assay
dc.subjectfemale
dc.subjectgene isolation
dc.subjectgene mutation
dc.subjectgenetic conservation
dc.subjecthuman
dc.subjecthuman cell
dc.subjectimmunoglobulin gene
dc.subjectimmunoglobulin variable region
dc.subjectinfection prevention
dc.subjectinoculation
dc.subjectmouse
dc.subjectnonhuman
dc.subjectpassive immunization
dc.subjectpriority journal
dc.subjectprotein assembly
dc.subjectprotein domain
dc.subjectprotein expression
dc.subjectprotein function
dc.subjectprotein glycosylation
dc.subjectRespiratory syncytial pneumovirus
dc.subjectreverse transcription polymerase chain reaction
dc.subjectvirus infection
dc.subjectvirus neutralization
dc.subjectvirus titration
dc.subjectwild type
dc.subjectAnimals
dc.subjectAntibodies, Monoclonal
dc.subjectAntibodies, Viral
dc.subjectEpitopes
dc.subjectHumans
dc.subjectImmunization, Passive
dc.subjectImmunoglobulin G
dc.subjectInjections, Intravenous
dc.subjectMice
dc.subjectMice, Inbred BALB C
dc.subjectNeutralization Tests
dc.subjectRespiratory Syncytial Virus Infections
dc.subjectRespiratory Syncytial Virus, Human
dc.subjectViral Fusion Proteins
dc.subjectChimaeriformes
dc.subjectHuman respiratory syncytial virus
dc.subjectHydrangea ringspot virus
dc.subjectMurinae
dc.subjectRodentia
dc.subjectSubgroup A
dc.titleProtection of mice against human respiratory syncytial virus by wild-type and aglycosyl mouse-human chimaeric lgG antibodies to subgroup-conserved epitopes on the G glycoprotein
dc.typeArticle
dc.rights.holderScopus
dc.identifier.bibliograpycitationJournal of General Virology. Vol 87, No.5 (2006), p.1267-1273
dc.identifier.doi10.1099/vir.0.81660-0
Appears in Collections:Scopus 1983-2021

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