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DC Field | Value | Language |
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dc.contributor.author | Mekseepralard C. | |
dc.contributor.author | Toms G.L. | |
dc.contributor.author | Routledge E.G. | |
dc.date.accessioned | 2021-04-05T04:32:22Z | - |
dc.date.available | 2021-04-05T04:32:22Z | - |
dc.date.issued | 2006 | |
dc.identifier.issn | 221317 | |
dc.identifier.other | 2-s2.0-33646146936 | |
dc.identifier.uri | https://ir.swu.ac.th/jspui/handle/123456789/15029 | - |
dc.identifier.uri | https://www.scopus.com/inward/record.uri?eid=2-s2.0-33646146936&doi=10.1099%2fvir.0.81660-0&partnerID=40&md5=e04892f06d8b0b390a488aa978284088 | |
dc.description.abstract | Monoclonal antibodies (mAbs) to conserved epitopes on the G glycoprotein of human respiratory syncytial virus (HRSV) subgroup A fail to neutralize the virus in cell culture in the absence of complement, but are protective in rodent models of infection. They may have potential as prophylactic agents in human infants. In order to investigate the role of Fc-dependent pathways in protection by one such antibody, 1 C2, the VH and VL genes were isolated by RT-PCR and assembled with human κ light-chain and human γ1 heavy-chain constant-region genes to form two mouse-human chimaeras, which were expressed in NSO cells. One of the chimaeras carried a wild-type γ1 chain, whilst the other had an aglycosyl mutation in the CH2 domain rendering the antibody defective in complement activation and FcγR binding. Whilst both chimaeric antibodies exhibited similar avidity for HRSV in ELISA, only the fully glycosylated wild type was capable of neutralizing the virus in the presence of complement. In mice passively immunized with either murine or wild-type γ1 chimaeric antibody, no virus could be recovered from the lungs 4 days after intranasal inoculation of HRSV. In mice immunized with the aglycosyl γ1 chimaera, however, virus was present in the lungs following challenge, although virus titres were significantly reduced compared with controls (P<0.005). These results indicate that the protective effect of this antibody is mediated by both Fc-dependent and Fc-independent pathway. © 2006 SGM. | |
dc.subject | alemtuzumab | |
dc.subject | epitope | |
dc.subject | Fc receptor | |
dc.subject | immunoglobulin Fc fragment | |
dc.subject | immunoglobulin G | |
dc.subject | immunoglobulin heavy chain | |
dc.subject | immunoglobulin light chain | |
dc.subject | immunoglobulin subclass | |
dc.subject | respiratory syncytial virus vaccine | |
dc.subject | thrombospondin | |
dc.subject | animal cell | |
dc.subject | animal experiment | |
dc.subject | animal model | |
dc.subject | animal tissue | |
dc.subject | antigen binding | |
dc.subject | article | |
dc.subject | chimera | |
dc.subject | complement activation | |
dc.subject | controlled study | |
dc.subject | enzyme linked immunosorbent assay | |
dc.subject | female | |
dc.subject | gene isolation | |
dc.subject | gene mutation | |
dc.subject | genetic conservation | |
dc.subject | human | |
dc.subject | human cell | |
dc.subject | immunoglobulin gene | |
dc.subject | immunoglobulin variable region | |
dc.subject | infection prevention | |
dc.subject | inoculation | |
dc.subject | mouse | |
dc.subject | nonhuman | |
dc.subject | passive immunization | |
dc.subject | priority journal | |
dc.subject | protein assembly | |
dc.subject | protein domain | |
dc.subject | protein expression | |
dc.subject | protein function | |
dc.subject | protein glycosylation | |
dc.subject | Respiratory syncytial pneumovirus | |
dc.subject | reverse transcription polymerase chain reaction | |
dc.subject | virus infection | |
dc.subject | virus neutralization | |
dc.subject | virus titration | |
dc.subject | wild type | |
dc.subject | Animals | |
dc.subject | Antibodies, Monoclonal | |
dc.subject | Antibodies, Viral | |
dc.subject | Epitopes | |
dc.subject | Humans | |
dc.subject | Immunization, Passive | |
dc.subject | Immunoglobulin G | |
dc.subject | Injections, Intravenous | |
dc.subject | Mice | |
dc.subject | Mice, Inbred BALB C | |
dc.subject | Neutralization Tests | |
dc.subject | Respiratory Syncytial Virus Infections | |
dc.subject | Respiratory Syncytial Virus, Human | |
dc.subject | Viral Fusion Proteins | |
dc.subject | Chimaeriformes | |
dc.subject | Human respiratory syncytial virus | |
dc.subject | Hydrangea ringspot virus | |
dc.subject | Murinae | |
dc.subject | Rodentia | |
dc.subject | Subgroup A | |
dc.title | Protection of mice against human respiratory syncytial virus by wild-type and aglycosyl mouse-human chimaeric lgG antibodies to subgroup-conserved epitopes on the G glycoprotein | |
dc.type | Article | |
dc.rights.holder | Scopus | |
dc.identifier.bibliograpycitation | Journal of General Virology. Vol 87, No.5 (2006), p.1267-1273 | |
dc.identifier.doi | 10.1099/vir.0.81660-0 | |
Appears in Collections: | Scopus 1983-2021 |
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