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Please use this identifier to cite or link to this item: https://ir.swu.ac.th/jspui/handle/123456789/15025
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dc.contributor.authorChaivisuthangkura P.
dc.contributor.authorPhattanapaijitkul P.
dc.contributor.authorThammapalerd N.
dc.contributor.authorRukpratanporn S.
dc.contributor.authorLongyant S.
dc.contributor.authorSithigorngul W.
dc.contributor.authorSithigorngul P.
dc.date.accessioned2021-04-05T04:32:21Z-
dc.date.available2021-04-05T04:32:21Z-
dc.date.issued2006
dc.identifier.issn1660934
dc.identifier.other2-s2.0-33645284994
dc.identifier.urihttps://ir.swu.ac.th/jspui/handle/123456789/15025-
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-33645284994&doi=10.1016%2fj.jviromet.2005.11.012&partnerID=40&md5=c5e0707783a8d802cbcc15d672a9f139
dc.description.abstractThe VP19 gene encoding a structural envelope protein of white spot syndrome virus was cloned into an expression vector and introduced into E. coli. The objective was to produce a recombinant VP19 structural protein. After induction, the recombinant VP19 protein (rVP19) was produced, purified by SDS-PAGE and used for immunization of Swiss mice for polyclonal antibody production. The mouse anti rVP19 antiserum had specific immunoreactivity to the viral antigen in WSSV infected Penaeus monodon as verified by immunohistochemistry and Western blot. The production of monoclonal antibodies against this rVP19 may be useful in order to combine with anti-VP28 monoclonal antibodies for enhancing the sensitivity of various WSSV serological assays. © 2006 Elsevier B.V. All rights reserved.
dc.subjectenvelope protein
dc.subjectmaltose binding protein
dc.subjectmonoclonal antibody
dc.subjectpolyclonal antibody
dc.subjectprotein vp 19
dc.subjectrecombinant protein
dc.subjectstructural protein
dc.subjectunclassified drug
dc.subjectarticle
dc.subjectimmunohistochemistry
dc.subjectmouse
dc.subjectnonhuman
dc.subjectpolyacrylamide gel electrophoresis
dc.subjectpolymerase chain reaction
dc.subjectpriority journal
dc.subjectWestern blotting
dc.subjectWhite spot syndrome virus
dc.subjectAnimals
dc.subjectAntibodies, Monoclonal
dc.subjectAntibodies, Viral
dc.subjectBlotting, Western
dc.subjectDNA, Viral
dc.subjectElectrophoresis, Polyacrylamide Gel
dc.subjectEscherichia coli
dc.subjectEthidium
dc.subjectGenes, Viral
dc.subjectImmunohistochemistry
dc.subjectMice
dc.subjectPenaeidae
dc.subjectPlasmids
dc.subjectRecombinant Proteins
dc.subjectViral Envelope Proteins
dc.subjectVirology
dc.subjectWhite spot syndrome virus 1
dc.subjectPenaeus monodon
dc.subjectShrimp white spot syndrome virus
dc.titleDevelopment of a polyclonal antibody specific to VP19 envelope protein of white spot syndrome virus (WSSV) using a recombinant protein preparation
dc.typeArticle
dc.rights.holderScopus
dc.identifier.bibliograpycitationJournal of Virological Methods. Vol 133, No.2 (2006), p.180-184
dc.identifier.doi10.1016/j.jviromet.2005.11.012
Appears in Collections:Scopus 1983-2021

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