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https://ir.swu.ac.th/jspui/handle/123456789/15025| Title: | Development of a polyclonal antibody specific to VP19 envelope protein of white spot syndrome virus (WSSV) using a recombinant protein preparation |
| Authors: | Chaivisuthangkura P. Phattanapaijitkul P. Thammapalerd N. Rukpratanporn S. Longyant S. Sithigorngul W. Sithigorngul P. |
| Keywords: | envelope protein maltose binding protein monoclonal antibody polyclonal antibody protein vp 19 recombinant protein structural protein unclassified drug article immunohistochemistry mouse nonhuman polyacrylamide gel electrophoresis polymerase chain reaction priority journal Western blotting White spot syndrome virus Animals Antibodies, Monoclonal Antibodies, Viral Blotting, Western DNA, Viral Electrophoresis, Polyacrylamide Gel Escherichia coli Ethidium Genes, Viral Immunohistochemistry Mice Penaeidae Plasmids Recombinant Proteins Viral Envelope Proteins Virology White spot syndrome virus 1 Penaeus monodon Shrimp white spot syndrome virus |
| Issue Date: | 2006 |
| Abstract: | The VP19 gene encoding a structural envelope protein of white spot syndrome virus was cloned into an expression vector and introduced into E. coli. The objective was to produce a recombinant VP19 structural protein. After induction, the recombinant VP19 protein (rVP19) was produced, purified by SDS-PAGE and used for immunization of Swiss mice for polyclonal antibody production. The mouse anti rVP19 antiserum had specific immunoreactivity to the viral antigen in WSSV infected Penaeus monodon as verified by immunohistochemistry and Western blot. The production of monoclonal antibodies against this rVP19 may be useful in order to combine with anti-VP28 monoclonal antibodies for enhancing the sensitivity of various WSSV serological assays. © 2006 Elsevier B.V. All rights reserved. |
| URI: | https://ir.swu.ac.th/jspui/handle/123456789/15025 https://www.scopus.com/inward/record.uri?eid=2-s2.0-33645284994&doi=10.1016%2fj.jviromet.2005.11.012&partnerID=40&md5=c5e0707783a8d802cbcc15d672a9f139 |
| ISSN: | 1660934 |
| Appears in Collections: | Scopus 1983-2021 |
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