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Title: Dopamine transporter immunoreactive terminals in the bovine pineal gland
Authors: Phansuwan-Pujito P.
Boontem P.
Chetsawang B.
Ebadi M.
Govitrapong P.
Keywords: dopamine beta monooxygenase
dopamine transporter
messenger RNA
tyrosine 3 monooxygenase
animal tissue
cellular distribution
controlled study
corpus striatum
dopaminergic nerve cell
nerve cell
nerve ending
pineal body
priority journal
protein expression
protein localization
reverse transcription polymerase chain reaction
substantia nigra
tissue distribution
Dopamine beta-Hydroxylase
Dopamine Plasma Membrane Transport Proteins
Nerve Endings
Nerve Fibers
Pineal Gland
Presynaptic Terminals
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger
Tyrosine 3-Monooxygenase
Issue Date: 2006
Abstract: The dopaminergic system has been proposed to be one of the innervations in controlling the mammalian pineal gland function. The dopamine receptors have been characterized in the pineal and the biphasic effects of dopamine on melatonin production have been demonstrated. Recently, the site of dopamine transporter (DAT), a plasma membrane transport protein of dopaminergic neuron, also has been characterized in the bovine pineal gland. The aim of the present study was to identify the dopaminergic innervation in the bovine pineal gland. The localization and expression of DAT have been performed by using an immunohistochemical method and a reverse transcriptase polymerase chain reaction (RT-PCR) technique. DAT-immunoreactivity was found in the nerve terminals throughout the gland, but not in pinealocytes or neuronal-like cells. Some DAT-immunoreactive nerve fibers were observed along the pineal stalk. DAT mRNA product from RT-PCR was found in the bovine substantia nigra, but not in the pineal gland. The colocalization of DAT with tyrosine hydroxylase (TH) or dopamine beta hydroxylase (DBH) immunoreactivities was observed in nerve terminals. However, no colocalization of DAT with DBH was found in some terminals/fibers. The present results showed the central dopaminergic innervation in the bovine pineal gland distinctively from noradrenergic nerve fibers, and their perikarya origin was located possibly outside of the gland. © 2006 Elsevier Ireland Ltd. All rights reserved.
ISSN: 3043940
Appears in Collections:Scopus 1983-2021

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