Please use this identifier to cite or link to this item: https://ir.swu.ac.th/jspui/handle/123456789/14999
ชื่อเรื่อง: Development of monoclonal antibodies for simple identification of Vibrio alginolyticus
ผู้แต่ง: Sithigorngul W.
Rengpipat S.
Tansirisittikul A.
Rukpratanporn S.
Longyant S.
Chaivisuthangkura P.
Sithigorngul P.
Keywords: monoclonal antibody
Antibodies
Bacteria
Cloning
Diseases
Immunology
antibody
bacterial disease
bacterium
crustacean
identification method
immunization
immunoassay
rodent
animal tissue
article
bacterium identification
controlled study
Gram negative infection
immunohistochemistry
mouse
nonhuman
shrimp
Vibrio alginolyticus
Vibrio cholerae
Vibrio harveyi
Vibrio mimicus
Vibrio parahaemolyticus
Vibrio vulnificus
Animals
Antibodies, Monoclonal
Antibody Specificity
Blotting, Western
Immunohistochemistry
Mice
Shellfish
Vibrio alginolyticus
Vibrio Infections
Animalia
Bacteria (microorganisms)
Decapoda (Crustacea)
Penaeus monodon
Vibrio alginolyticus
Vibrio cholerae
Vibrio fluvialis
Vibrio harveyi
Vibrio mimicus
Vibrio parahaemolyticus
Vibrio penaeicida
Vibrio vulnificus
วันที่เผยแพร่: 2006
บทคัดย่อ: Aims: The present study was aimed to produce monoclonal antibodies (MAbs) for simple and specific identification of Vibrio alginolyticus infection in shrimp. Methods and Results: Mice were immunized with heat killed V. alginolyticus four times at 2-week intervals. The best response mouse was used for spleen donor in hybridoma production. Screening of hybridoma clones producing desired antibodies was performed by dot blotting against V. alginolyticus and other bacterial species, Western blotting and immunohistochemistry of infected shrimp tissues. Four groups of MAbs were obtained; the first group of MAbs demonstrated their limited specificity only to V. alginolyticus used for immunization, while the second and the third groups recognized all three isolates of V. alginolyticus used for testing. The fourth group of MAbs bound to all three isolates of V. alginolyticus and also recognized Vibrio parahaemolyticus, Vibrio harveyi, Vibrio fluvialis and Vibrio vulnificus but did not bind to Vibrio mimicus, Vibrio cholerae, Vibrio penaeicida and other bacterial species tested. MAbs in groups 1, 2 and 3 were able to use for the detection of bacterial infection in the tissues by means of immunohistochemistry. Conclusions: MAbs specific to V. alginolyticus was produced. These MAbs can be used for specific identification of the bacteria by simple 'dot blotting' method and immunohistochemistry. Significance and Impact of the Study: This study demonstrated an immunological tool that can be used for simple and accurate identification of V. alginolyticus as well as for the diagnosis of V. alginolyticus infection in animals. This immunological tool can replace costly and laborious biochemical tests. © 2006 The Authors.
URI: https://ir.swu.ac.th/jspui/handle/123456789/14999
https://www.scopus.com/inward/record.uri?eid=2-s2.0-33748617109&doi=10.1111%2fj.1472-765X.2006.01969.x&partnerID=40&md5=425edc5f496fd3237bfcbfc0b6c3e789
ISSN: 2668254
Appears in Collections:Scopus 1983-2021

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