Please use this identifier to cite or link to this item: https://ir.swu.ac.th/jspui/handle/123456789/14961
ชื่อเรื่อง: Effects of vitrification procedures on subsequent development and ultrastructure of in vitro-matured swamp buffalo (Bubalus bubalis) oocytes
ผู้แต่ง: Boonkusol D.
Faisaikarm T.
Dinnyes A.
Kitiyanant Y.
Keywords: ethylene glycol
animal cell
article
blastocyst
buffalo
cell survival
cell ultrastructure
cell vacuole
controlled study
correlation analysis
cryopreservation
feasibility study
membrane damage
microvillus
mitochondrial membrane
mitochondrion
nonhuman
oocyte
oocyte development
oocyte maturation
survival rate
transmission electron microscopy
vitrification
zona pellucida
Animals
Buffaloes
Cell Survival
Cryopreservation
Ethylene Glycol
Female
Microscopy, Electron
Mitochondria
Oocytes
Surface Properties
Bubalus bubalis
Sugarcane streak virus
วันที่เผยแพร่: 2007
บทคัดย่อ: The purpose of the present study was to investigate the effects of two vitrification procedures on developmental capacity and ultrastructural changes of matured swamp buffalo oocytes. In vitro-matured oocytes were vitrified by using 35 and 40% ethylene glycol as vitrification solution for solid surface vitrification (SSV) and in-straw vitrification (ISV), respectively. Survival rate of vitrified-warmed oocytes, evaluated on the basis of ooplasm homogeneity, oolemma integrity and zona pellucida intactness, as well as parthenogenetic blastocyst rates of vitrified-warmed oocytes were significantly higher with SSV (89.3 and 13.6%, respectively) than ISV (81.8 and 5.5%, respectively). However, they were still significantly lower than that of control oocytes (100 and 34.2%, respectively). For examining the ultrastructural changes, fresh, VS-exposed (ISV and SSV), and vitrified-warmed (ISV and SSV) oocytes were processed for transmission electron microscopy. In VS-exposed oocytes, reduction of microvilli abundance and damage of mitochondrial membrane were found only in the ISV group. In vitrified-warmed oocytes, however, it was clear that both methods of vitrification induced profound ultrastructural modifications to microvilli, mitochondria, oolemma and cortical granules as well as to the size and position of vesicles. Damaged mitochondria were, however, more abundant in ISV vitrified oocytes than in SSV vitrified oocytes, which correlated with the developmental data, showing the superiority of the SSV method. The present study demonstrated the feasibility of vitrification of in vitro-matured swamp buffalo oocytes. © CSIRO 2007.
URI: https://ir.swu.ac.th/jspui/handle/123456789/14961
https://www.scopus.com/inward/record.uri?eid=2-s2.0-33846630322&doi=10.1071%2fRD06097&partnerID=40&md5=28f9dc3194429efcbc09863b9ba22721
ISSN: 10313613
Appears in Collections:Scopus 1983-2021

Files in This Item:
There are no files associated with this item.


Items in SWU repository are protected by copyright, with all rights reserved, unless otherwise indicated.