Please use this identifier to cite or link to this item: https://ir.swu.ac.th/jspui/handle/123456789/14957
ชื่อเรื่อง: Proteome and immunome of pathogenic Leptospira spp. revealed by 2DE and 2DE-immunoblotting with immune serum
ผู้แต่ง: Sakolvaree Y.
Maneewatch S.
Jiemsup S.
Klaysing B.
Tongtawe P.
Srimanote P.
Saengjaruk P.
Banyen S.
Tapchaisri P.
Chonsa-nguan M.
Chaicumpa W.
Keywords: 3 [(3 cholamidopropyl)dimethylammonio] 1 propanesulfonic acid
3 oxoacyl acyl carrier protein synthase
acetyl coenzyme A acetyltransferase
bacterial protein
bacterial vaccine
chaperone
chaperonin
DNA directed DNA polymerase gamma
elongation factor Ts
elongation factor Tu
endopeptidase Clp
flavoprotein
gene product
glutamate ammonia ligase
glyceraldehyde 3 phosphate dehydrogenase
leptospirosis vaccine
lipoprotein
nicotinamide adenine dinucleotide (phosphate) transhydrogenase
NifU like protein
outer membrane protein
oxidoreductase
proteinase inhibitor
proteome
spermidine synthase
sphingomyelin phosphodiesterase
succinate dehydrogenase
succinyl coenzyme A synthetase
transfer RNA methyltransferase
unclassified drug
urea
article
bacterial cell
blood analysis
cell disruption
controlled study
drug design
drug targeting
electron transport
energy transfer
genetic transcription and translation
homogenate
immune response
immunoblotting
Leptospira
Leptospira borgpetersenii
Leptospira interrogans
leptospirosis
male
mouse
nonhuman
nucleotide sequence
protein analysis
protein degradation
protein folding
protein function
protein targeting
signal transduction
species difference
two dimensional gel electrophoresis
ultrasound
Amino Acid Sequence
Animals
Antigens, Bacterial
Blotting, Western
Electrophoresis, Gel, Two-Dimensional
Leptospira
Leptospira interrogans serovar icterohaemorrhagiae
Leptospirosis
Male
Mice
Mice, Inbred BALB C
Molecular Sequence Data
Proteome
Proteomics
วันที่เผยแพร่: 2007
บทคัดย่อ: In this study, proteomes of two pathogenic Leptospira spp., namely L. interrogans, serogroup Icterohaemorrhagiae, serovar Copenhageni and L. borgpetersenii, serogroup Tarassovi, serovar Tarassovi, were revealed by using two dimensional gel electrophoresis (2DE)-based-proteomics. Bacterial cells were disrupted in a lysis buffer containing 30 mM Tris, 2 M thiourea, 7 M urea, 4% CHAPS, 2% IPG buffer pH 3-10 and protease inhibitors and then subjected to sonication in order to solubilize as much as possible the bacterial proteins. The 2DE-separated components of both Leptospira homogenates were blotted individually onto membranes and antigenic components (immunomes) were revealed by probing the blots with immune serum of a mouse readily immunized with the homogenate of L. interrogans, serogroup Icterohaemorrhagiae, serovar Copenhageni. The immunogenic proteins of the two pathogenic Leptospira spp. could be grouped into 10 groups. These are: 1) proteins involved in the bacterial transcription and translation including beta subunit transcription anti-termination protein of DNA polymerase III, elongation factors Tu and Ts, and tRNA (guanine-N1)-methyltransferase; 2) proteins functioning as enzymes for metabolisms and nutrient acquisition including acetyl-Co-A acetyltransferase, putative glutamine synthetase, glyceraldehyde-3-phospahte dehydrogenase, NifU-like protein, 3-oxoacyl-(acyl-carrier-protein) reductase, oxidoreductase, sphingomyelinase C precursor, spermidine synthase, beta subunit of succinyl-CoA synthetase, and succinate dehydrogenase iron-sulfur subunit; 3) proteins/enzymes necessary for energy and electron transfer, i.e. electron transfer flavoprotein, and proton-translocating transhydrogenase; 4) enzymes for degradation of misfolded proteins, i.e. ATP-dependent Cip protease; 5) molecular chaperone, i.e. 60 kDa chaperonin; 6) signal transduction system, i.e. response regulator; 7) protein involved in immune evasion in host, i.e. peroxiredoxin; 8) cell structure proteins including MreB (cytoskeletal) and flagellin/ periplasmic flagellin; 9) lipoproteins/outer membrane proteins: LipL32, LipL41, LipL45 and OmpL1; and 10) various hypothetical proteins. Many immunogenic proteins are common to both Leptospira spp. These proteins not only are the diagnostic targets but also have potential as candidates of a broad spectrum leptospirosis vaccine especially the surface exposed components which should be vulnerable to the host immune effector factors.
URI: https://ir.swu.ac.th/jspui/handle/123456789/14957
https://www.scopus.com/inward/record.uri?eid=2-s2.0-34250897884&partnerID=40&md5=3de8bb1a6da33498cf7753d12ffff957
ISSN: 0125877X
Appears in Collections:Scopus 1983-2021

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