Please use this identifier to cite or link to this item: https://ir.swu.ac.th/jspui/handle/123456789/14877
ชื่อเรื่อง: Specific monoclonal antibodies raised against Taura syndrome virus (TSV) capsid protein VP3 detect TSV in single and dual infections with white spot syndrome virus (WSSV)
ผู้แต่ง: Longyant S.
Poyoi P.
Chaivisuthangkura P.
Tejangkura T.
Sithigorngul W.
Sithigorngul P.
Rukpratanporn S.
Keywords: capsid protein
monoclonal antibody
recombinant protein
virus antibody
antibody
bacterium
crustacean
gene expression
protein
animal
antibody specificity
article
biosynthesis
Escherichia coli
genetics
immunology
isolation and purification
mouse
Penaeidae
Picornavirus
reproducibility
virology
Western blotting
White spot syndrome virus
Animals
Antibodies, Monoclonal
Antibodies, Viral
Antibody Specificity
Blotting, Western
Capsid Proteins
Escherichia coli
Mice
Penaeidae
Picornaviridae
Recombinant Proteins
Reproducibility of Results
White spot syndrome virus 1
Decapoda (Crustacea)
Escherichia coli
Hepatopancreatic parvovirus of penaeid shrimp
Litopenaeus vannamei
Miridae
Monodon baculovirus
Mus
Parvovirus
Shrimp white spot syndrome virus
Taura syndrome virus
Yellow head virus
วันที่เผยแพร่: 2008
บทคัดย่อ: The gene sequence encoding VP3 capsid protein of Taura syndrome virus (TSV) was cloned into pGEX-6P-1 expression vector and transformed into Escherichia coli BL21. After induction, recombinant GST-VP3 (rVP3) fusion protein was obtained and further purified by electro-elution before use in immunizing Swiss mice for production of monoclonal antibodies (MAb). One MAb specific to glutathione-S-transferase (GST) and 6 MAb specific to VP3 were selected using dot blotting and Western blotting. MAb specific to VP3 could be used to detect natural TSV infections in farmed whiteleg shrimp Penaeus vannamei by dot blotting and Western blotting, without cross reaction to shrimp tissues or other shrimp viruses, such as white spot syndrome virus (WSSV), yellow head virus (YHV), monodon baculovirus (MBV) and hepatopancreatic parvovirus (HPV). These MAb were also used together with those specific for WSSV to successfully detect TSV and WSSV in dual infections in farmed P. vannamei. © Inter-Research 2008.
URI: https://ir.swu.ac.th/jspui/handle/123456789/14877
https://www.scopus.com/inward/record.uri?eid=2-s2.0-42249096772&doi=10.3354%2fdao01885&partnerID=40&md5=53d0fb9d8fe6ecf5731fbb3be41b6a1a
ISSN: 1775103
Appears in Collections:Scopus 1983-2021

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