Please use this identifier to cite or link to this item: https://ir.swu.ac.th/jspui/handle/123456789/14864
ชื่อเรื่อง: Preventive effects of genistein on leukocyte adhesion in femur venules and on bone-loss induced in ovariectomized female rats
ผู้แต่ง: Kasiyaphat A.
Siriviriyakul P.
Patumraj S.
Keywords: alkaline phosphatase
dimethyl sulfoxide
estradiol
genistein
osteocalcin
alkaline phosphatase blood level
animal experiment
animal tissue
article
bone blood flow
bone mineral
chemoluminescence
controlled study
drug effect
female
femur
fluorescence
immunoassay
laser Doppler flowmetry
leukocyte adherence
microcirculation
nonhuman
ossification
osteolysis
ovariectomy
rat
Animals
Bone and Bones
Bone Density
Cell Adhesion
Disease Models, Animal
Female
Femur
Genistein
Leukocytes
Microscopy, Video
Ovariectomy
Phytoestrogens
Rats
Rats, Wistar
Venules
วันที่เผยแพร่: 2008
บทคัดย่อ: This study was aimed to examine effects of genistein on leukocyte adhesion in femur microcirculation in relation to bone-loss induced in ovariectomized female rats. Sixty-four female Wistar rats were divided into 4 groups: sham (daily treated with vehicle; DMSO, sc; 100 μl/day), ovariectomized rat treated with vehicle (OVXveh), 17β-estradiol treated-ovariectomized rat (OVXE2, 5 μg/kg/day, sc) and genistein treated-ovariectomized rat (0.25 mg/kg/day, sc; OVXgen). One and three weeks after the ovariectomy, blood flow perfusion (BF) in femur tissue was measured using laser Doppler flowmetry. Leukocyte adhesion in femur venules (15-30 μm in diameter) of each group was evaluated by intravital fluorescence videomicroscopy. The bone mineral content (BMC) was measured and expressed in terms of ratio of ash-to-dry matter weight. Serum osteocalcin and alkaline phosphatase levels were determined using chemiluminescence immunoassay. In both one and three week-OVXveh, leukocyte adhesion increased significantly, compared to their age-matched sham groups, but it decreased significantly in OVXgen, compared to OVXveh (p<0.05). In three week-OVXgen, both BF and BMC increased significantly, but osteocalcin and alkaline phosphatase levels decreased, compared to those of three week-OVXveh. In conclusion, genistein supplementation could effectively prevent bone-loss and microvascular endothelial dysfunction induced by estrogen deficiency. © 2008 - IOS Press and the authors. All rights reserved.
URI: https://ir.swu.ac.th/jspui/handle/123456789/14864
https://www.scopus.com/inward/record.uri?eid=2-s2.0-42049099042&partnerID=40&md5=8ed8987a8397a89741e46ee4438402a4
ISSN: 13860291
Appears in Collections:Scopus 1983-2021

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