Please use this identifier to cite or link to this item: https://ir.swu.ac.th/jspui/handle/123456789/14829
ชื่อเรื่อง: Immunomodulatory effects of cadmium and Gynostemma pentaphyllum herbal tea on rat splenocyte proliferation
ผู้แต่ง: Suntararuks S.
Yoopan N.
Rangkadilok N.
Worasuttayangkurn L.
Nookabkaew S.
Satayavivad J.
Keywords: cadmium
immunologic factor
plant extract
animal
article
B lymphocyte
beverage
cell division
chemistry
cytology
drug effect
food contamination
Gynostemma
immunology
male
rat
spleen
Sprague Dawley rat
T lymphocyte
Animals
B-Lymphocytes
Beverages
Cadmium
Cell Division
Food Contamination
Gynostemma
Immunologic Factors
Male
Plant Extracts
Rats
Rats, Sprague-Dawley
Spleen
T-Lymphocytes
Gynostemma pentaphyllum
Phytolaccaceae
Rattus
วันที่เผยแพร่: 2008
บทคัดย่อ: Gynostemma pentaphyllum Makino (GP) is a herbal tea widely grown in Southeast Asia. However, this herbal tea can be contaminated with some heavy metals, especially cadmium (Cd), from agricultural areas, which may affect human health. The objective of this study is to evaluate the immunomodulatory effects of Cd contaminated in GP herbal tea and inorganic Cd on rat splenocytes. Rats were divided into groups and treated with drinking water (control), high CdCl2 in drinking water (HCd; 0.05 mg/L), GP herbal tea containing 0.05 mg/L Cd (GP-HCd) for 4 months, low CdCl2 in drinking water (LCd; 0.006 mg/L), and GP herbal tea containing 0.006 mg/L Cd (GP-LCd) for 6 months. After the treatments, Cd accumulation in organs and blood was detected by using a graphite furnace atomic absorption spectrophotometer. In spleen, HCd-treated rats had 4-fold higher Cd accumulations than GP-HCd-treated rats. Cd accumulation in liver and kidney in the HCd group also increased significantly. There were no significant changes in total leucocyte and lymphocyte counts; however, these parameters tended to decrease slightly in LCd, GP-LCd, and GP-HCd groups. The HCd group (ex vivo) significantly produced suppressive effects on T cell mitogen-induced splenocyte proliferation, with 1 μg/mL Con A and PHA-P. In addition, 0.5 μg/mL PWM-induced B cell proliferation, through T cell functions, was also significantly inhibited by HCd as compared to the control group, while GP-HCd had no effects. However, both GP-LCd- and LCd-treated rats had a slight increase in Con A-stimulated splenocyte proliferation. This study indicated that high Cd contamination in drinking water alone had suppressive effects on T cell functions, but these effects could not be found with the same Cd level contamination in GP herbal tea. © 2008 American Chemical Society.
URI: https://ir.swu.ac.th/jspui/handle/123456789/14829
https://www.scopus.com/inward/record.uri?eid=2-s2.0-54349118020&doi=10.1021%2fjf801062z&partnerID=40&md5=4fe1445118ddc438415a3ace41facf28
ISSN: 218561
Appears in Collections:Scopus 1983-2021

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