Expression and purification of 30 kilodalton protein antigen of ARA -Burkholderia pseudomallei

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DC Field	Value	Language
dc.contributor.author	Rungpanich U.
dc.contributor.author	Pongsunk S.
dc.contributor.author	Ezaki T.
dc.contributor.author	Ekpo P.
dc.date.accessioned	2021-04-05T04:31:56Z	-
dc.date.available	2021-04-05T04:31:56Z	-
dc.date.issued	2009
dc.identifier.issn	1251562
dc.identifier.other	2-s2.0-63149121299
dc.identifier.uri	https://ir.swu.ac.th/jspui/handle/123456789/14792	-
dc.identifier.uri	https://www.scopus.com/inward/record.uri?eid=2-s2.0-63149121299&partnerID=40&md5=45207395b128cb31217ab0f33faa9bd0
dc.description.abstract	The 30 kDa protein of B. pseudomallei is found in virulent Ara- but not avirulent Ara+ strain. The gene was cloned in Escherichia coli JM105 employing pInIII-C2 vector. The open reading frame was 870 nucleotides with a guanine plus cytosine content of 69.9%. Arginine was the most abundant amino acid in the protein, having a PI of 12.65. Nucleotide sequence of the gene was 96% identical to B. pseudomallei 1710b chromosome II sequence CP000125.1, encoding an oxidoreductase of the short chain dehydrogenase/ reductase family. The 30 kDa antigen was expressed as a maltose-fusion protein with a yield of 5.25 mg/l of bacterial culture.
dc.title	Expression and purification of 30 kilodalton protein antigen of ARA -Burkholderia pseudomallei
dc.type	Article
dc.rights.holder	Scopus
dc.identifier.bibliograpycitation	Southeast Asian Journal of Tropical Medicine and Public Health. Vol 40, No.2 (2009), p.295-301
Appears in Collections:	Scopus 1983-2021

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