Please use this identifier to cite or link to this item:
https://ir.swu.ac.th/jspui/handle/123456789/14788
Title: | IL4 gene polymorphism and previous malaria experiences manipulate anti-Plasmodium falciparum antibody isotype profiles in complicated and uncomplicated malaria |
Authors: | Tangteerawatana P. Perlmann H. Hayano M. Kalambaheti T. Troye-Blomberg M. Khusmith S. |
Keywords: | immunoglobulin E antibody immunoglobulin G antibody interleukin 4 immunoglobulin class immunoglobulin E immunoglobulin G interleukin 4 adolescent adult aged article controlled study disease severity disease transmission endemic disease enzyme linked immunosorbent assay female genetic variability genotype human major clinical study malaria male Plasmodium falciparum restriction fragment length polymorphism single nucleotide polymorphism antibody production blood genetic variability genetics hospitalization immunology malaria falciparum middle aged parasitology polymerase chain reaction Adolescent Adult Aged Antibody Formation Enzyme-Linked Immunosorbent Assay Female Genetic Variation Genotype Humans Immunoglobulin E Immunoglobulin G Immunoglobulin Isotypes Interleukin-4 Malaria, Falciparum Male Middle Aged Plasmodium falciparum Polymerase Chain Reaction Polymorphism, Restriction Fragment Length Severity of Illness Index Young Adult |
Issue Date: | 2009 |
Abstract: | Abstract. Background. The IL4-590 gene polymorphism has been shown to be associated with elevated levels of anti-Plasmodium falciparum IgG antibodies and parasite intensity in the malaria protected Fulani of West Africa. This study aimed to investigate the possible impact of IL4-590C/T polymorphism on anti-P. falciparum IgG subclasses and IgE antibodies levels and the alteration of malaria severity in complicated and uncomplicated malaria patients with or without previous malaria experiences. Methods. Anti-P.falciparum IgG subclasses and IgE antibodies in plasma of complicated and uncomplicated malaria patients with or without previous malaria experiences were analysed using ELISA. IL4-590 polymorphisms were genotyped using RFLP-PCR. Statistical analyses of the IgG subclass levels were done by Oneway ANOVA. Genotype differences were tested by Chi-squared test. Results. The IL4-590T allele was significantly associated with anti-P. falciparum IgG3 antibody levels in patients with complicated (P = 0.031), but not with uncomplicated malaria (P = 0.622). Complicated malaria patients with previous malaria experiences carrying IL4-590TT genotype had significantly lower levels of anti-P. falciparum IgG3 (P = 0.0156), while uncomplicated malaria patients with previous malaria experiences carrying the same genotype had significantly higher levels (P = 0.0206) compared to their IL4-590 counterparts. The different anti-P. falciparum IgG1 and IgG3 levels among IL4 genotypes were observed. Complicated malaria patients with previous malaria experiences tended to have lower IgG3 levels in individuals carrying TT when compared to CT genotypes (P = 0.075). In contrast, complicated malaria patients without previous malaria experiences carrying CC genotype had significantly higher anti-P. falciparum IgG1 than those carrying either CT or TT genotypes (P = 0.004, P = 0.002, respectively). Conclusion. The results suggest that IL4-590C or T alleles participated differently in the regulation of anti-malarial antibody isotype profiles in primary and secondary malaria infection and, therefore, could play an important role in alteration of malaria severity. © 2009 Tangteerawatana et al; licensee BioMed Central Ltd. |
URI: | https://ir.swu.ac.th/jspui/handle/123456789/14788 https://www.scopus.com/inward/record.uri?eid=2-s2.0-74549211889&doi=10.1186%2f1475-2875-8-286&partnerID=40&md5=836747fa18b1a6c0624b217eb7b4f82e |
ISSN: | 14752875 |
Appears in Collections: | Scopus 1983-2021 |
Files in This Item:
There are no files associated with this item.
Items in SWU repository are protected by copyright, with all rights reserved, unless otherwise indicated.