Please use this identifier to cite or link to this item: https://ir.swu.ac.th/jspui/handle/123456789/14768
ชื่อเรื่อง: Spermidine-induced apoptosis via reactive oxygen species generation and caspase 3 activation in mouse P19 embryonal carcinoma cells
ผู้แต่ง: Nusuetrong P.
Suwannasual U.
Meksuriyen D.
Keywords: 3 (4,5 dimethyl 2 thiazolyl) 2,5 diphenyltetrazolium bromide
caspase 3
dichlorodihydrofluorescein diacetate
hoe 33342
propidium iodide
protein Bax
protein bcl 2
reactive oxygen metabolite
spermidine
animal cell
apoptosis
article
cell cycle G0 phase
cell cycle G1 phase
cell viability
chromatin condensation
controlled study
cytotoxicity test
DNA fragmentation
down regulation
embryo
embryonal carcinoma stem cell
enzyme activation
flow cytometry
fluorescence analysis
incubation time
mouse
nonhuman
protein expression
staining
toxicity testing
upregulation
Western blotting
วันที่เผยแพร่: 2010
บทคัดย่อ: The present study was to investigate the effects of spermidine-induced apoptosis in mouse PI 9 embryonal carcinoma cells. The cell viability using MTT (3-[4,5-dimefhyl-2-thiazolyl]-2,5-diphenyl-2H-tetrazolium bromide) assay showed that spermidine decreased cell viability after 24-h incubation in a concentration-dependent manner with an IC50 value of approximately 20 μM. Using Hoechst33342 staining, chromatin condensation and apoptotic bodies in PI 9 cells treated with spermidine were observed. Moreover, flow cytometric analysis stained with propidium iodide increased the accumulation of DNA fragmentation in the pre GJGi phase, indicating apoptotic cell death, which was accompanied by the generation of reactive oxygen species (ROS) as detected with the fluorescence probe 2',7'-dichlorofluorescin diacetate (DCFH-DA). Western blot analysis revealed that spermidine-induced apoptosis was associated with an increase of Bax and caspase 3 expression, meanwhile the expression of Bcl-2 was decreased. The results suggest that spermidine-induced PI 9 apoptotic cell death is involving ROS, Bcl-2 family as well as caspase 3. © 2010 Asian Network for Scientific Information.
URI: https://ir.swu.ac.th/jspui/handle/123456789/14768
https://www.scopus.com/inward/record.uri?eid=2-s2.0-77958029206&doi=10.3923%2fijp.2010.903.909&partnerID=40&md5=ce033b1b2a92203c6f9c32e735b97801
ISSN: 18117775
Appears in Collections:Scopus 1983-2021

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