Please use this identifier to cite or link to this item: https://ir.swu.ac.th/jspui/handle/123456789/14764
ชื่อเรื่อง: Authentication of Coscinium fenestratum among the other Menispermaceae plants prescribed in Thai folk medicines
ผู้แต่ง: Watthanachaiyingcharoen R.
Komatsu K.
Zhu S.
Vajragupta O.
Leelamanit W.
Keywords: internal transcribed spacer
ribosome DNA
ribosome RNA
arcangelisia flava
article
controlled study
coscinium fenestratum
electrophoresis
Fibraurea tinctoria
gene sequence
Menispermaceae
nonhuman
nucleotide sequence
polymerase chain reaction
restriction fragment length polymorphism
traditional medicine
Base Sequence
DNA, Ribosomal Spacer
Drug Contamination
Electrophoresis
Medicine, East Asian Traditional
Menispermaceae
Plant Components, Aerial
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length
RNA, Ribosomal, 18S
Sequence Analysis, DNA
วันที่เผยแพร่: 2010
บทคัดย่อ: In Ayurveda and Thai traditional medicines, material from Coscinium fenestratum is commonly prescribed as active ingredients with diverse therapeutic purposes. However, C. fenestratum is also a seriously endangered medicinal liana. Thus, its crude material is very rare and is being substituted with substances from Arcangelisia flava or Fibraurea tinctoria (Menispermaceae), which have high morphological similarity. In this current study, nuclear 18S ribosomal RNA (rRNA) gene and nuclear ribosomal DNA internal transcribed spacer (ITS) gene sequences with the polymerase chain reaction-restriction fragment length polymorphisms (PCR-RFLPs) technique were exploited to identify these three species. The nuclear 18S rRNA gene sequences of C. fenestratum, A. flava, and F. tinctoria consisted of 1809, 1805, and 1809 base pairs (bps), respectively, while their ITS gene regions were 694, 622, and 631 bps in length, respectively. The 18S rRNA gene of C. fenestratum digested with SmaI restriction enzyme displayed the electrophoresis profile of 729 and 790 bps; for A. flava and F. tinctoria, the digested products showed fragments of 1519 bps. Although the ITS gene regions of A. flava and F. tinctoria had unrecognized sequences with SalI, the SalI-digested ITS of C. fenestratum exhibited fragments of approximately 599 bp. Thus, the 18S rRNA gene and ITS gene sequences with PCR-RFLPs were proven to be powerful molecular markers for identifying C. fenestratum and distinguishing it from the other two Menispermaceae plants. © 2010 Pharmaceutical Society of Japan.
URI: https://ir.swu.ac.th/jspui/handle/123456789/14764
https://www.scopus.com/inward/record.uri?eid=2-s2.0-75149167072&doi=10.1248%2fbpb.33.91&partnerID=40&md5=24aef10b3f9c83c96893dc262d1736bd
ISSN: 9186158
Appears in Collections:Scopus 1983-2021

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