Please use this identifier to cite or link to this item: https://ir.swu.ac.th/jspui/handle/123456789/14730
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dc.contributor.authorChaivisuthangkura P.
dc.contributor.authorLongyant S.
dc.contributor.authorHajimasalaeh W.
dc.contributor.authorSridulyakul P.
dc.contributor.authorRukpratanporn S.
dc.contributor.authorSithigorngul P.
dc.date.accessioned2021-04-05T03:36:49Z-
dc.date.available2021-04-05T03:36:49Z-
dc.date.issued2010
dc.identifier.issn1660934
dc.identifier.other2-s2.0-73049092057
dc.identifier.urihttps://ir.swu.ac.th/jspui/handle/123456789/14730-
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-73049092057&doi=10.1016%2fj.jviromet.2009.11.007&partnerID=40&md5=431a96c5058e31fc6d27793cc6926379
dc.description.abstractTaura syndrome virus (TSV) is one of the major pathogens causing mortality in the whiteleg shrimp, Litopenaeus vannamei. In this study, the gene sequence encoding the VP2 capsid protein (40 kDa) of TSV was cloned into pMAL-C2 expression vector. Five monoclonal antibodies (MAbs) were produced against the VP2 capsid protein, which was expressed heterologously in the form of a fusion protein with maltose binding protein and called MBP-VP2. All MAbs belonged to the IgG1 subclass and could bind MBP-VP2 at 400-800 pg/spot in immuno-dot blot assays. The MAbs could detect VP2 both in extracts from shrimp infected naturally in western blotting and dot blotting and in shrimp tissues in immunohistochemistry. Additionally, these MAbs did not exhibit cross-reactivity to extracts from uninfected shrimp or shrimp infected with several other common viruses. However, the dot blot assay sensitivity for TSV was approximately 10,000 times lower than that of one step RT-PCR. The MAb TSV2-88 specific to VP2 obtained in this study demonstrated an approximately twofold higher sensitivity than that of the MAb specific to VP3 from a previous study. In immunohistochemistry, the MAb TSV2-88 specific to VP2 demonstrated stronger immunoreactivity than the MAb TSV3-601 specific to VP3. A combination of the VP2 and VP3 MAbs could be used to more easily detect TSV infections in field samples of L. vannamei with better sensitivity and fidelity than using a single MAb. © 2009 Elsevier B.V. All rights reserved.
dc.subjecthybrid protein
dc.subjectimmunoglobulin G1
dc.subjectmaltose binding protein
dc.subjectmonoclonal antibody
dc.subjectprotein VP2
dc.subjectprotein VP3
dc.subjectanimal tissue
dc.subjectantigen binding
dc.subjectarticle
dc.subjectcell lysate
dc.subjectcontrolled study
dc.subjectcross reaction
dc.subjectdot hybridization
dc.subjectgene sequence
dc.subjectimmunoblotting
dc.subjectimmunodetection
dc.subjectimmunohistochemistry
dc.subjectimmunoreactivity
dc.subjectnonhuman
dc.subjectpriority journal
dc.subjectprotein analysis
dc.subjectprotein expression
dc.subjectreal time polymerase chain reaction
dc.subjectRNA virus
dc.subjectRNA virus infection
dc.subjectsensitivity and specificity
dc.subjectshrimp
dc.subjectTaura syndrome virus
dc.subjectvirus detection
dc.subjectvirus recombinant
dc.subjectWestern blotting
dc.subjectAnimals
dc.subjectAntibodies, Monoclonal
dc.subjectAntibodies, Viral
dc.subjectCapsid Proteins
dc.subjectCloning, Molecular
dc.subjectCross Reactions
dc.subjectDicistroviridae
dc.subjectImmunoblotting
dc.subjectImmunohistochemistry
dc.subjectPenaeidae
dc.subjectRecombinant Fusion Proteins
dc.subjectReverse Transcriptase Polymerase Chain Reaction
dc.subjectRNA Virus Infections
dc.subjectSensitivity and Specificity
dc.subjectSequence Analysis, DNA
dc.subjectDecapoda (Crustacea)
dc.subjectLitopenaeus vannamei
dc.subjectMiridae
dc.subjectTaura syndrome virus
dc.titleImproved sensitivity of Taura syndrome virus immunodetection with a monoclonal antibody against the recombinant VP2 capsid protein
dc.typeArticle
dc.rights.holderScopus
dc.identifier.bibliograpycitationJournal of Virological Methods. Vol 163, No.2 (2010), p.433-439
dc.identifier.doi10.1016/j.jviromet.2009.11.007
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