Please use this identifier to cite or link to this item: https://ir.swu.ac.th/jspui/handle/123456789/14717
ชื่อเรื่อง: Detection of the shrimp pathogenic bacteria, Vibrio harveyi, by a quartz crystal microbalance-specific antibody based sensor
ผู้แต่ง: Buchatip S.
Ananthanawat C.
Sithigorngul P.
Sangvanich P.
Rengpipat S.
Hoven V.P.
Keywords: Alkanethiols
Binding efficiency
Bovine serum albumins
Carbodiimides
Carboxyl groups
Cross-reactivity
Gold electrodes
Hydroxyl-terminated
Mixed SAM
Monoclonal antibodies (mAb)
N-hydroxysuccinimide
Pathogenic bacterium
Penaeus monodon
Shrimp culture
Vibrio parahaemolyticus
Vibrio vulnificus
Working range
Bacteriology
Body fluids
Chemical detection
Immunosensors
Monoclonal antibodies
Oxide minerals
Piezoelectric devices
Quartz
Quartz crystal microbalances
Self assembled monolayers
Surface plasmon resonance
Bacteria
วันที่เผยแพร่: 2010
บทคัดย่อ: An immunosensor based on a quartz crystal microbalance (QCM) was developed for detection of Vibrio harveyi (V. harveyi), a pathogenic bacteria causing morbidity and even a high level of fatality in commercial shrimp cultures, including Penaeus monodon. Immobilization of a monoclonal antibody (MAb) against V. harveyi onto the gold electrode of a QCM plate involved a three-step procedure: (1) the formation of a self-assembled monolayer (SAM) of a carboxyl-terminated alkanethiol, (2) activation of the carboxyl groups by N-hydroxysuccinimide (NHS) and 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC), and (3) attachment of the MAb to the activated carboxyl groups. The developed QCM-based immunosensor could be used for the detection of V. harveyi in a working range of 103-107 CFU/mL, with no detectable cross-reactivity to Vibrio vulnificus and Vibrio parahaemolyticus. Controlling the density of surface-immobilized MAb, by the formation of mixed SAMs containing hydroxyl-terminated alkanethiol in combination with the treatment of 1% (w/v) bovine serum albumin (BSA) as a blocking reagent, can significantly improve the binding efficiency of the targeted bacteria. © 2009 Elsevier B.V. All rights reserved.
URI: https://ir.swu.ac.th/jspui/handle/123456789/14717
https://www.scopus.com/inward/record.uri?eid=2-s2.0-77149134952&doi=10.1016%2fj.snb.2009.12.003&partnerID=40&md5=551ba7bef5f35c7986374b46df1a3695
ISSN: 9254005
Appears in Collections:Scopus 1983-2021

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