Please use this identifier to cite or link to this item: https://ir.swu.ac.th/jspui/handle/123456789/14555
ชื่อเรื่อง: High transfection efficiency of cationic lipids with asymmetric acyl-Cholesteryl hydrophobic tails
ผู้แต่ง: Radchatawedchakoon W.
Krajarng A.
Niyomtham N.
Watanapokasin R.
Yingyongnarongkul B.-E.
Keywords: cationic lipids
DNA delivery
Hydrophobic tails
Nonviral vectors
solid-phase synthesis
DNA
Drug therapy
Gene transfer
Hydrophobicity
Liposomes
Mammals
Nucleic acids
Phospholipids
Gene therapy
1,2 dioleoyl glycero 3 phosphatidyl ethanolamine
1,2-dioleoyl-glycero-3-phosphatidyl ethanolamine
cation
cholesterol
DNA
lipid
liposome
phosphatidylethanolamine
animal
article
cell strain HEK293
cell survival
chemical phenomena
chemistry
dog
drug effect
genetic transfection
human
metabolism
tumor cell line
Animals
Cations
Cell Line, Tumor
Cell Survival
Cholesterol
DNA
Dogs
HEK293 Cells
Humans
Hydrophobic and Hydrophilic Interactions
Lipids
Liposomes
Phosphatidylethanolamines
Transfection
วันที่เผยแพร่: 2011
บทคัดย่อ: The ability of a nonviral gene delivery system to overcome extra- and intracellular barriers is a critical issue for the future clinical applications of gene therapy. In recent years much effort has been focused on the development of a variety of DNA carriers, and cationic liposomes have become the most common nonviral gene delivery system. One hundred and eighty novel cationic lipids with asymmetric acyl-cholesteryl hydrophobic tails were synthesized by parallel solid-phase chemistry. The liposomes were prepared and gel retardation assays were used to study the binding efficiency between the prepared liposome and the DNA. Transfection efficiencies of the lipids were evaluated against various mammalian cells, such as human embryonic kidney (HEK293), human cervical adenocarcinoma (HeLa), canine osteosarcoma (D17), colorectal adenocarcinoma (COLO 205), and human prostate adenocarcinoma (PC3) cells. The lipids with an acyl portion at the terminal part of the polyamine backbone exhibited higher transfection efficiency than those with the acyl portion as an internal part of the backbone. These compounds also showed higher transfection efficiency and lower cytotoxicity than the commercially available agents, Effectene, DOTAP, and DC-Chol. © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
URI: https://ir.swu.ac.th/jspui/handle/123456789/14555
https://www.scopus.com/inward/record.uri?eid=2-s2.0-79952156409&doi=10.1002%2fchem.201001622&partnerID=40&md5=5a6d32ebe9219bf7a470cd5320dbdf0e
ISSN: 9476539
Appears in Collections:Scopus 1983-2021

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