Please use this identifier to cite or link to this item: https://ir.swu.ac.th/jspui/handle/123456789/14464
ชื่อเรื่อง: Purification, crystallization and preliminary X-ray analysis of recombinant betaine aldehyde dehydrogenase 2 (OsBADH2), a protein involved in jasmine aroma, from Thai fragrant rice (Oryza sativa L.)
ผู้แต่ง: Kuaprasert B.
Silprasit K.
Horata N.
Khunrae P.
Wongpanya R.
Boonyalai N.
Vanavichit A.
Choowongkomon K.
Keywords: betaine aldehyde dehydrogenase
recombinant protein
article
chemistry
enzymology
isolation and purification
rice
X ray crystallography
Betaine-Aldehyde Dehydrogenase
Crystallography, X-Ray
Oryza sativa
Recombinant Proteins
Escherichia coli
Jasminum
Oryza sativa
วันที่เผยแพร่: 2011
บทคัดย่อ: Fragrant rice (Oryza sativa L.) betaine aldehyde dehydrogenase 2 (OsBADH2) is a key enzyme in the synthesis of fragrance aroma compounds. The extremely low activity of OsBADH2 in catalyzing the oxidation of acetaldehyde is believed to be crucial for the accumulation of the volatile compound 2-acetyl-1-pyrroline (2AP) in many scented plants, including fragrant rice. Recombinant fragrant rice OsBADH2 was expressed in Escherichia coli as an N-terminal hexahistidine fusion protein, purified using Ni Sepharose affinity chromatography and crystallized using the microbatch method. Initial crystals were obtained within 24 h using 0.1 M Tris pH 8.5 with 30%(w/v) PEG 4000 and 0.2 M magnesium chloride as the precipitating agent at 291 K. Crystal quality was improved when the enzyme was cocrystallized with NAD +. Improved crystals were grown in 0.1 M HEPES pH 7.4, 24%(w/v) PEG 4000 and 0.2 M ammonium chloride and diffracted to beyond 2.95 Å resolution after being cooled in a stream of N2 immediately prior to X-ray diffraction experiments. The crystals belonged to space group C2221, with unit-cell parameters a = 66.03, b = 183.94, c = 172.28 Å. An initial molecular-replacement solution has been obtained and refinement is in progress. © 2011 International Union of Crystallography All rights reserved.
URI: https://ir.swu.ac.th/jspui/handle/123456789/14464
https://www.scopus.com/inward/record.uri?eid=2-s2.0-80955133098&doi=10.1107%2fS1744309111030971&partnerID=40&md5=53c0ffa85fbd831a514688ab679b6c60
ISSN: 17443091
Appears in Collections:Scopus 1983-2021

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