Please use this identifier to cite or link to this item:
https://ir.swu.ac.th/jspui/handle/123456789/14384
ชื่อเรื่อง: | Development of a one-step immunochromatographic strip test using gold nanoparticles for the rapid detection of Salmonella typhi in human serum |
ผู้แต่ง: | Preechakasedkit P. Pinwattana K. Dungchai W. Siangproh W. Chaicumpa W. Tongtawe P. Chailapakul O. |
Keywords: | Analysis time Control zones Fiber membranes Gold Nanoparticles Human serum Immunochromatographic strip test Immunochromatographic strips Limit of detection Lower detection limit Naked-eye Nanoparticle conjugate Nitrocellulose membranes Rapid detection Salmonella typhi Sandwich immunoassay Specific binding Antibodies Antigens Body fluids Cost accounting Glass fibers Gold Immunology Nanoparticles Nitrocellulose Salmonella Testing bacterium antibody glass fiber gold nanoparticle immunoglobulin G antibody pyroxylin antibody labeling antibody specificity antigen binding article bacterium detection blood analysis coated particle controlled study diagnostic accuracy enzyme immunoassay human immunoaffinity chromatography immunoblotting immunochromatographic strip test intermethod comparison limit of detection nonhuman process development reaction time Salmonella typhi serum Bacterial Load Biosensing Techniques Blood Chemical Analysis Equipment Design Equipment Failure Analysis Gold Humans Immunochromatography Nanoparticles Nanotechnology Reagent Strips Reproducibility of Results Salmonella typhi Sensitivity and Specificity Accounting Antibodies Binding Cellulose Nitrate Glass Fibers Gold Membranes Salmonella typhi |
วันที่เผยแพร่: | 2012 |
บทคัดย่อ: | An immunochromatographic strip test using gold nanoparticles was developed for the rapid detection of Salmonella typhi (S. typhi) in human serum. The strip test based on the principle of sandwich immunoassay by the specific binding of antigens from S. typhi O901 and antibody of S. typhi O901 on a nitrocellulose membrane. Antibody-gold nanoparticle conjugate was used as the label and was coated onto a glass fiber membrane, which was used as a conjugate pad. To create a test and control zone, antibody of S. typhi O901 and an anti-IgG were dotted on the nitrocellulose membrane, respectively. Positive samples were displayed as red dots at the test and control zones of the nitrocellulose membrane, while negative samples resulted in a red dot only in the control zone. The limit of detection (LOD) was found to be 1.14×10 5cfumL -1, which could be visually detected by the naked eye within 15min. This strip test provided a lower detection limit and analysis time than a dot blot immunoassay (8.88×10 6cfumL -1 for LOD and 110min for reaction time). In addition, our immunochromatographic strip test was employed to detect S. typhi in human serum effectively, with high accuracy. This strip test offers great promise for a rapid, simple and low-cost analysis of S. typhi. © 2011 Elsevier B.V. |
URI: | https://ir.swu.ac.th/jspui/handle/123456789/14384 https://www.scopus.com/inward/record.uri?eid=2-s2.0-84455208100&doi=10.1016%2fj.bios.2011.10.031&partnerID=40&md5=84938ebbe31e1075304c2f4a1b4a6841 |
ISSN: | 9565663 |
Appears in Collections: | Scopus 1983-2021 |
Files in This Item:
There are no files associated with this item.
Items in SWU repository are protected by copyright, with all rights reserved, unless otherwise indicated.