Please use this identifier to cite or link to this item: https://ir.swu.ac.th/jspui/handle/123456789/14305
ชื่อเรื่อง: Monoclonal antibodies against extra small virus show that it co-localizes with Macrobrachium rosenbergii nodavirus
ผู้แต่ง: Longyant S.
Senapin S.
Sanont S.
Wangman P.
Chaivisuthangkura P.
Rukpratanporn S.
Sithigorngul P.
Keywords: monoclonal antibody
virus RNA
antibody
electrokinesis
enzyme activity
immunoassay
muscle
pathogenicity
prawn culture
protein
viral disease
animal
article
hybridoma
immunology
mouse
Nodavirus
Palaemonidae
physiology
virology
Animals
Antibodies, Monoclonal
Hybridomas
Mice
Nodaviridae
Palaemonidae
RNA, Viral
Decapoda (Crustacea)
Escherichia coli
Macrobrachium rosenbergii
Macrobrachium rosenbergii nodavirus
Miridae
Mus
Penaeidae
White tip die-back phytoplasma
วันที่เผยแพร่: 2012
บทคัดย่อ: The capsid protein (CP) gene of extra small virus (XSV) expressed in Escherichia coli as a 42 kDa glutathione S- ENGLtransferase (GST)-ENGLfusion protein (GST-XCP) or a 20 kDa His6-fusion protein (His6-XCP) were purified by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), combined, and used to immunize Swiss mice to produce monoclonal antibodies (MAbs). Using dot blot, Western blot, and immunohistochemistry (IHC) methods, 4 MAbs specific to the XSV CP detected XSV in the freshwater prawn Macrobrachium rosenbergii without crossreaction to host proteins or to proteins of Macrobrachium rosenbergii nodavirus (MrNV) or 5 of the most pathogenic viruses of penaeid shrimp. In dot blots, the combined MAbs could detect down to ~10 to 20 fmol μl-1 of purified GST-XCP protein, which was somewhat more sensitive compared to any single MAb. Used in conjunction with an MrNV-specific MAb, white tail disease (WTD) was diagnosed more effectively. However, the sensitivity at which the combined 4 MAbs detected XSV CP was 1000-fold lower than XSV RNA detected by RT-PCR. IHC analysis of M. rosenbergii tissue sections using the MAbs showed XSV infection to co-localize at variable loads with MrNV infection in heart and muscle cells as well as cells of connective tissues in the hepatopancreas. Since XSV histopathology remained prominent in tissues of some prawns in which MAb reactivity for MrNV was low compared to MAb reactivity for XSV, XSV might play some role in WTD severity. © Inter-Research 2012.
URI: https://ir.swu.ac.th/jspui/handle/123456789/14305
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84866232879&doi=10.3354%2fdao02482&partnerID=40&md5=9a7864c7e61f76b092b36541462447cd
ISSN: 1775103
Appears in Collections:Scopus 1983-2021

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