Please use this identifier to cite or link to this item: https://ir.swu.ac.th/jspui/handle/123456789/14294
ชื่อเรื่อง: Growth inhibition and chemosensitization of human carcinoma cells by human serum albumin-coated liposomal antisense oligodeoxyribonucleotide against bcl-2
ผู้แต่ง: Weecharangsan W.
Lee R.J.
Keywords: alpha tocopheryl polyethylene glycol 1000 succinate
antisense oligodeoxynucleotide
dimethyldioctadecyl ammonium bromide
doxorubicin
egg phosphatidylcholine
human serum albumin
liposome
messenger RNA
protein bcl 2
unclassified drug
article
carcinoma cell
cell growth
cell strain KB
chemosensitization
concentration response
controlled study
cytotoxicity
drug coating
drug delivery system
genetic transfection
growth inhibition
human
human cell
incubation time
mouth carcinoma
priority journal
protein expression
Antibiotics, Antineoplastic
Cell Growth Processes
Cell Line, Tumor
Dose-Response Relationship, Drug
Doxorubicin
Gene Expression Regulation, Neoplastic
Humans
KB Cells
Lipids
Liposomes
Mouth Neoplasms
Oligodeoxyribonucleotides, Antisense
Proto-Oncogene Proteins c-bcl-2
RNA, Messenger
Serum Albumin
Time Factors
วันที่เผยแพร่: 2012
บทคัดย่อ: Previous study has shown human serum albumin (HSA) coated liposomes can deliver bcl-2 antisense oligodeoxyribonucleotide (ODN) into KB carcinoma cells, and decrease bcl-2 mRNA and protein expression level. In the current study, cell growth inhibition and chemosensitization of KB cells were evaluated. Liposomes composed of dimethyldioctadecyl ammonium bromide/egg phosphatidylcholine/ α-tocopheryl polyethylene glycol 1000 succinate (58:40:2 molar ratio) complexed with bcl-2 antisense ODN and coated with HSA were examined for cell growth inhibition and sensitization to a commonly used chemotherapeutic drug, doxorubicin. HSA-coated liposomeODN complexes effectively inhibited cell growth in the range of ODN concentration of 0.457.2 M. Upon posttreatment with doxorubicin, the cytotoxicity was further significantly increased compared to the ODN complexes alone. The cytotoxicity was dependent on antisense ODN concentration, incubation time and doxorubicin concentration, and relatively independent on HSA concentration. This study suggests that HSA-coated liposomes are effective delivery vehicles for antisense ODN with potential therapeutic application and can be effectively combined with doxorubicin. © 2012 Informa Healthcare USA, Inc.
URI: https://ir.swu.ac.th/jspui/handle/123456789/14294
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84866256041&doi=10.3109%2f10717544.2012.714810&partnerID=40&md5=49c52723b72986b90604e875f73414ff
ISSN: 10717544
Appears in Collections:Scopus 1983-2021

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