Please use this identifier to cite or link to this item: https://ir.swu.ac.th/jspui/handle/123456789/14271
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dc.contributor.authorBoonburapong B.
dc.contributor.authorLaloknam S.
dc.contributor.authorYamada N.
dc.contributor.authorIncharoensakdi A.
dc.contributor.authorTakabe T.
dc.date.accessioned2021-04-05T03:33:56Z-
dc.date.available2021-04-05T03:33:56Z-
dc.date.issued2012
dc.identifier.issn9168451
dc.identifier.other2-s2.0-84866649295
dc.identifier.urihttps://ir.swu.ac.th/jspui/handle/123456789/14271-
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-84866649295&doi=10.1271%2fbbb.120309&partnerID=40&md5=14d9233fe04df6f1dec602021ba4cb79
dc.description.abstractGlutamate is a major free amino acid in cyanobacteria, but its transport properties remain largely unknown. In this study, we found that a halotolerant cyanobacterium, Aphanothece halophytica, contained a sodium dependent glutamate transporter (ApGltS). The deduced amino acid sequence of ApGltS exhibited low homology (18-19% identity) to GltS from Synechocystis sp. PCC 6803 (slr1145) and Escherichia coli. The predicted ApGltS consisted of 476 amino acid residues with a molecular weight of 50,976 Da. As analysed by hydropathy profiling, ApGltS contains 11 transmembrane segments. The ApgltS gene was isolated and expressed in E. coli ME9107, which is deficient in glutamate uptake. ME9107, expressing ApGltS, took up glutamate and its rates increased with increasing concentrations of NaCl. Kinetics studies revealed that ApGltS is a high-affinity glutamate transporter with a Km of about 5μM. The presence of 0.5M NaCl in the assay medium increased Vmax by about 3-fold. Competition experiments revealed that glutamate, glutamine, aspartate, and asparagine inhibited glutamate uptake. The level of mRNA for ApgltS was higher in A. halophytica grown at high salinity. Under high salinity conditions supplemented with glutamate, A. halophytica showed a significant increase in intracellular glycine betaine.
dc.subjectAmino acid residues
dc.subjectAmino acid sequence
dc.subjectAmino acid uptake
dc.subjectAphanothece halophytica
dc.subjectAspartates
dc.subjectCyanobacterium
dc.subjectE. coli
dc.subjectEnhanced growth
dc.subjectFree amino acids
dc.subjectGlutamate transporters
dc.subjectGlycine betaine
dc.subjectHalotolerant
dc.subjectHigh salinity
dc.subjectKinetics studies
dc.subjectSalt stresss
dc.subjectSynechocystis sp. PCC 6803
dc.subjectTransmembrane segments
dc.subjectEscherichia coli
dc.subjectHardwoods
dc.subjectSalinity measurement
dc.subjectSodium
dc.subjectSodium chloride
dc.subjectTransport properties
dc.subjectAmino acids
dc.subjectamino acid transporter
dc.subjectbacterial protein
dc.subjectbetaine
dc.subjectcotransporter
dc.subjectEscherichia coli protein
dc.subjectgltS protein, E coli
dc.subjectglutamate transporter
dc.subjectglutamic acid
dc.subjectmessenger RNA
dc.subjectrecombinant protein
dc.subjectsodium chloride
dc.subjectamino acid sequence
dc.subjectarticle
dc.subjectbiosynthesis
dc.subjectcyanobacterium
dc.subjectdrug effect
dc.subjectenzyme specificity
dc.subjectEscherichia coli
dc.subjectgenetics
dc.subjectgrowth, development and aging
dc.subjectkinetics
dc.subjectmetabolism
dc.subjectmolecular genetics
dc.subjectmolecular weight
dc.subjectphylogeny
dc.subjectphysiology
dc.subjectprotein tertiary structure
dc.subjectsalinity
dc.subjectsalt tolerance
dc.subjectsequence homology
dc.subjectAmino Acid Sequence
dc.subjectAmino Acid Transport System X-AG
dc.subjectAmino Acid Transport Systems, Acidic
dc.subjectBacterial Proteins
dc.subjectBetaine
dc.subjectCyanobacteria
dc.subjectEscherichia coli
dc.subjectEscherichia coli Proteins
dc.subjectGlutamic Acid
dc.subjectKinetics
dc.subjectMolecular Sequence Data
dc.subjectMolecular Weight
dc.subjectPhylogeny
dc.subjectProtein Structure, Tertiary
dc.subjectRecombinant Proteins
dc.subjectRNA, Messenger
dc.subjectSalinity
dc.subjectSalt-Tolerance
dc.subjectSequence Homology, Amino Acid
dc.subjectSodium Chloride
dc.subjectSubstrate Specificity
dc.subjectSymporters
dc.subjectAphanothece halophytica
dc.subjectCyanobacteria
dc.subjectEscherichia coli
dc.subjectSynechocystis sp. PCC 6803
dc.titleSodium-dependent uptake of glutamate by novel ApGltS enhanced growth under salt stress of halotolerant cyanobacterium aphanothece halophytica
dc.typeArticle
dc.rights.holderScopus
dc.identifier.bibliograpycitationBioscience, Biotechnology and Biochemistry. Vol 76, No.9 (2012), p.1702-1707
dc.identifier.doi10.1271/bbb.120309
Appears in Collections:Scopus 1983-2021

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