Please use this identifier to cite or link to this item: https://ir.swu.ac.th/jspui/handle/123456789/14214
ชื่อเรื่อง: The effect of temperature of eye irrigation solution to reduce corneal endothelial cell loss during phacoemulsification: An in vitro model study
ผู้แต่ง: Uthaisang-Tanechpongtamb W.
Limtrakarn W.
Reepolmaha S.
Keywords: balanced salt solution
air conditioning
animal cell
animal model
article
cell death
cell growth
cell isolation
cell structure
cell survival
controlled study
cornea endothelium
corneal endothelial cell loss
eye lavage
freezing
heat
nonhuman
phacoemulsification
swine
temperature
Analysis of Variance
Animals
Cell Death
Cell Survival
Cells, Cultured
Corneal Endothelial Cell Loss
Endothelium, Corneal
Ophthalmic Solutions
Phacoemulsification
Swine
Temperature
Therapeutic Irrigation
วันที่เผยแพร่: 2012
บทคัดย่อ: Objective: To compare the efficiency of eye irrigation solution (balanced salt solution: BSS) stored in a refrigerator and air-conditioned room to reduce the number of corneal endothelial cell loss during the process of phacoemulsification. Material and Method: Porcine corneal endothelial cells (PCEC) isolated from porcine eyes were used as a model. The porcine eyes were obtained from a local slaughterhouse within 6 h after death and PCEC were cultured as standard method. The percentage of cell survival was evaluated by MTT assay. Results: The PCEC was successfully grown in the standard culturing system. The growth curve of PCEC demonstrated that log phase was reached in 3-6 days. The evaluation of heat effect (40-60°C) to PCECsurvival was shown that cells death was found at 55 and 60°C which the percentage of cell survival reduced to 30% at 60°C, 180 sec. Further observation on the efficiency of BSS stored in different conditions, refrigeration (8°C) and air-conditioned room (25°C) to PCEC survival was revealed that the number of cell survival increased from 40% to almost 70% when using refrigerated BSS as irrigation solution, while from air-conditioned BSS only at 55%. Conclusion: From using PCEC as a model and mimic the process of irrigation in phacoemulsification, it was found that the eye irrigation solution (balanced salt solution: BSS) stored in the refrigerator had a better result in helping protection the corneal endothelial cell loss from heat damaged than BSS stored in air-conditioned room.
URI: https://ir.swu.ac.th/jspui/handle/123456789/14214
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84876941086&partnerID=40&md5=dc34a5404f2e01772b2fe4b42e3be9b1
ISSN: 1252208
Appears in Collections:Scopus 1983-2021

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