Please use this identifier to cite or link to this item: https://ir.swu.ac.th/jspui/handle/123456789/14151
ชื่อเรื่อง: Protective effect of Phyllanthus emblica fruit extract against hydrogen peroxide-induced endothelial cell death
ผู้แต่ง: Wongpradabchai S.
Chularojmontri L.
Phornchirasilp S.
Wattanapitayakul S.K.
Keywords: antioxidant
Emblica officinalis extract
flavonoid
hydrogen peroxide
immunoglobulin enhancer binding protein
protein kinase B
reactive oxygen metabolite
article
cardiovascular disease
cell death
cell viability
colorimetry
controlled study
cytotoxicity
Emblica officinalis
endothelium cell
fluorescence analysis
fruit
human cell
humna
oxidative stress
prophylaxis
protein phosphorylation
Western blotting
Analysis of Variance
Blotting, Western
Cell Death
Cells, Cultured
Endothelial Cells
Humans
Hydrogen Peroxide
NF-kappa B
Oxidative Stress
Phosphatidylinositol 3-Kinases
Phosphorylation
Phyllanthus emblica
Plant Extracts
Reactive Oxygen Species
วันที่เผยแพร่: 2013
บทคัดย่อ: Numerous antioxidants from natural products have been shown to lower ROS levels and enhance vascular endothelial function. The fruits of Phyllanthus emblica are well-known in possessing antioxidative properties but its role and mechanisms in the protection of vascular endothelial cells from ROS damage have not yet been established. The present study was aimed to determine the possible protective effect of P. emblica fruit extract (PE) on human EA.hy926 endothelial cell death induced by hydrogen peroxide (H2O2) and PE protective mechanisms. Following incubation of endothelial cells with 300 microM H2O2 for 2 h, cell viability was decreased to 50.65 ± 0.94% and intracellular ROS levels was increased to 159.01% ± 6.27% as measured by MTT assay and DCF fluorescent intensity, respectively. Cytotoxic effect of PE was not observed in the range of 0.1 to 100 microM. Pretreatment with PE (20 to 100 microg/mL) for 48 h significantly ameliorated the cytotoxic effect of H2O2 and attenuated the excessive intracellular ROS formation in endothelial cells. In addition, western blot analysis revealed that PE pretreatment (40 microg/L) induced Akt phosphorylation but did not activate NF-kappaB pathway. These findings suggest that PE could effectively protect human endothelial cell death induced by H2O2 via modification of ROSrelated mechanism along with activation of PI3K/Akt pathway. However, the value of this plant in vivo needs further investigations in supporting them to be developed as nutraceuticals for cardiovascular disease prevention.
URI: https://ir.swu.ac.th/jspui/handle/123456789/14151
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84876837988&partnerID=40&md5=54a23ff2771ec22c499410cf6eb6e494
ISSN: 1252208
Appears in Collections:Scopus 1983-2021

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