Please use this identifier to cite or link to this item: https://ir.swu.ac.th/jspui/handle/123456789/14130
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dc.contributor.authorKhunvirojpanich M.
dc.contributor.authorWattanasirichaigoon S.
dc.contributor.authorPradidarcheep W.
dc.date.accessioned2021-04-05T03:33:15Z-
dc.date.available2021-04-05T03:33:15Z-
dc.date.issued2013
dc.identifier.issn1252208
dc.identifier.other2-s2.0-84876853950
dc.identifier.urihttps://ir.swu.ac.th/jspui/handle/123456789/14130-
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-84876853950&partnerID=40&md5=fda4d34f954f3b0a54efd0e1e836d68e
dc.description.abstractBackground: In order to detoxify ammonia, mammalian livers use carbamoyl phosphate synthetase (CPS) and glutamine synthetase (GS) for conversion into respective non-toxic urea and glutamine. CPS is expressed in the periportal hepatocytes whereas GS is contained in the pericentral hepatocytes. Objective: To examine the expressional changes of CPS and GS in the liver being induced to become cirrhotic by hepatotoxin thioacetamide (TAA). Material and Method: Twenty-five male Wistar rats were divided into 5 groups of 5 animals each. Group 1 was for control. Groups 2 to 5 were treated with 200 mg/kg TAA intraperitoneally three times weekly for 1, 2, 3 and 4 months respectively. The immunohistochemical technique was employed in order to elucidate the expression of CPS and GS in each animal group. Results: As centro-central fibrous bridging developed in the course of TAA treatment, expression of CPS declined dramatically and that of GS was no longer restricted to the pericentral hepatocytes. In month 4, CPS-positive hepatocytes were only found in some regenerative nodules, whereas GS expression became confined to the nodular periphery. Proper CPS staining required tissue fixation in a mixture of methanol, acetone and water (2:2:1 v/v) as opposed to 4% paraformaldehyde. Conclusion: In response to the hepatotoxin TAA, the liver attempts to regenerate by means of conserving persistent CPSpositive hepatocytes and rearranging GS-positive hepatocytes in response to vascular obstruction.
dc.subjectcarbamoyl phosphate synthase
dc.subjectglutamate ammonia ligase
dc.subjectthioacetamide
dc.subjectalanine aminotransferase blood level
dc.subjectalkaline phosphatase blood level
dc.subjectanimal experiment
dc.subjectanimal model
dc.subjectanimal tissue
dc.subjectarticle
dc.subjectaspartate aminotransferase blood level
dc.subjectcontrolled study
dc.subjectimmunohistochemistry
dc.subjectliver cell
dc.subjectliver cirrhosis
dc.subjectliver function test
dc.subjectliver regeneration
dc.subjectmale
dc.subjectmicroscopy
dc.subjectnonhuman
dc.subjectprotein expression
dc.subjectrat
dc.subjectAnalysis of Variance
dc.subjectAnimals
dc.subjectCarbamyl Phosphate
dc.subjectGlutamate-Ammonia Ligase
dc.subjectLiver Cirrhosis, Experimental
dc.subjectLiver Function Tests
dc.subjectMale
dc.subjectRats
dc.subjectRats, Wistar
dc.subjectThioacetamide
dc.titleExpressional changes of carbamoyl phosphate synthetase and glutamine synthetase in the liver of rat with thioacetamide-induced cirrhosis
dc.typeArticle
dc.rights.holderScopus
dc.identifier.bibliograpycitationJournal of the Medical Association of Thailand. Vol 96, No.SUPPL.1 (2013), p.S71-S77
Appears in Collections:Scopus 1983-2021

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