Please use this identifier to cite or link to this item: https://ir.swu.ac.th/jspui/handle/123456789/14125
ชื่อเรื่อง: Antioxidant effects of gamma-oryzanol on human prostate cancer cells
ผู้แต่ง: Klongpityapong P.
Supabphol R.
Supabphol A.
Keywords: antilipemic agent
antioxidant
catalase
gamma oryzanol
gamma-oryzanol
glutathione
malonaldehyde
messenger RNA
phenylpropionic acid derivative
apoptosis
article
cell proliferation
drug effect
enzyme linked immunosorbent assay
genetics
human
lipid peroxidation
male
metabolism
pathology
prostate tumor
real time polymerase chain reaction
reverse transcription polymerase chain reaction
tumor cell culture
Western blotting
Antioxidants
Apoptosis
Blotting, Western
Catalase
Cell Proliferation
Enzyme-Linked Immunosorbent Assay
Glutathione
Humans
Hypolipidemic Agents
Lipid Peroxidation
Male
Malondialdehyde
Phenylpropionates
Prostatic Neoplasms
Real-Time Polymerase Chain Reaction
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger
Tumor Cells, Cultured
วันที่เผยแพร่: 2013
บทคัดย่อ: Background: To assess the antioxidant effects of gamma-oryzanol on human prostate cancer cells. Materials and Methods: Cytotoxic activity of gamma-oryzanol on human DU145 and PC3 prostate cancer cells was determined by proliferation assay using 3-(4, 5-dimethylthiazol, 2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) reagent. mRNA levels of genes involved in the intracellular antioxidant system, superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX) and glutathione reductase (GSR) were determined by reverse transcription-polymerase chain reaction (RT-PCR). Cancer cell lysates were used to measure lipid peroxidation using thiobarbituric acid reactive substance (TBARS). Glutathione contents of the cell lysates were estimated by the reaction between sulfhydryl group of 5, 5'-dithio (bis) nitrobenzoic acid (DTNB) to produce a yellow-color of 5-thio-2-nitrobenzoic acid using colorimetric assay. Catalase activity was also analysed by examining peroxidative function. Protein concentration was estimated by Bradford's assay. Results: All concentrations of gamma-oryzanol, 0.1-2.0mg/ml, significantly inhibited cell growth in a dose- and time-dependent fashion in both prostate cancer cell lines, DU145 and PC3. Gene expression of catalase in DU145 and PC3 exposed to gamma-orizanol at 0.5mg/ml for 14 days was down regulated, while mRNA of GPX was also down regulated in PC3. The MDA and glutathione levels including catalase activity in the cell lysates of DU145 and PC3 treated with gamma-oryzanol 0.1 and 0.5mg/ml were generally decreased. Conclusions: This study highlighted effects of gamma-oryzanol via the down-regulation of antioxidant genes, catalase and GPX, not cytotoxic roles. This might be interesting for adjuvant chemotherapy to make prostate cancer cells more sensitive to free radicals. It might be useful for the reduction of cytotoxic agents and cancer chemoprevention.
URI: https://ir.swu.ac.th/jspui/handle/123456789/14125
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84887568262&doi=10.7314%2fAPJCP.2013.14.9.5421&partnerID=40&md5=15c67cf22c6ace3ff988759672450121
ISSN: 15137368
Appears in Collections:Scopus 1983-2021

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