Please use this identifier to cite or link to this item: https://ir.swu.ac.th/jspui/handle/123456789/14111
ชื่อเรื่อง: Pectin nanoparticle enhances cytotoxicity of methotrexate against hepG2 cells
ผู้แต่ง: Chittasupho C.
Jaturanpinyo M.
Mangmool S.
Keywords: methotrexate
nanoparticle
pectin
article
cancer cell culture
cytotoxicity
drug delivery system
drug release
drug structure
human
human cell
in vitro study
liver cancer
pH measurement
priority journal
Antimetabolites, Antineoplastic
Cytotoxins
Drug Delivery Systems
Hep G2 Cells
Humans
Methotrexate
Nanoparticles
Pectins
วันที่เผยแพร่: 2013
บทคัดย่อ: Objective: This work has aimed to develop methotrexate-conjugated pectin nanoparticle for delivering a cytotoxic drug to hepatic cancer cell. Methods: Methotrexate was conjugated to pectin by carbodiimide chemistry. Nanoparticles of pectin conjugated with methotrexate (MTX) were then fabricated by using ionotropic gelation. The size, shape and surface charge were characterized by dynamic light scattering and microscopic method. Cytotoxicity of MTX-pectin nanoparticle was monitored by MTT assay. Results: Methotrexate-pectin nanoparticle was successfully formulated. Drug release study indicated that MTX-NP exhibited sustained drug release at pH 7.4. Sustained release of methotrexate may enable methotrexate-pectin nanoparticle as a controlled drug delivery system. Cytotoxicity study confirmed the activity of the drug incorporated in nanoparticles. In addition, the cytotoxicity of methotrexate was increased when conjugated to pectin nanoparticles, compared to free methotrexate. Conclusions: This study verified that pectin can deliver methotrexate to hepatic cancer cell and provide sustained drug delivery. The cytotoxicity of methotrexate was enhanced when methotrexate was conjugated to pectin indicating the improved drug delivery to cancer cell. © 2013 Informa Healthcare USA, Inc.
URI: https://ir.swu.ac.th/jspui/handle/123456789/14111
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84872251182&doi=10.3109%2f10717544.2012.739214&partnerID=40&md5=c83dd3b9068374e9736d4da81ef1e914
ISSN: 10717544
Appears in Collections:Scopus 1983-2021

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