Please use this identifier to cite or link to this item: https://ir.swu.ac.th/jspui/handle/123456789/14074
ชื่อเรื่อง: Improvement of immunodetection of white spot syndrome virus using a monoclonal antibody specific for heterologously expressed icp11
ผู้แต่ง: Siriwattanarat R.
Longyant S.
Chaivisuthangkura P.
Wangman P.
Vaniksampanna A.
Sithigorngul P.
Keywords: Crangon crangon
Decapoda (Crustacea)
Escherichia coli
Litopenaeus vannamei
Mus
Penaeus
Shrimp white spot syndrome virus
วันที่เผยแพร่: 2013
บทคัดย่อ: The icp11 gene encoding the highly abundant DNA mimic protein of white spot syndrome virus (WSSV) was cloned into the pTYB1 and pGEX-6P-1 expression vectors and introduced into E. coli by transformation. After induction, C-terminally intein-tagged ICP11 (ICP11-intein) and N-terminally glutathione-S-transferase (GST)-tagged ICP11 (GST-ICP11) proteins with molecular masses of 64 and 35 kDa were obtained. These proteins were purified by SDS-PAGE and used for immunization of Swiss mice for monoclonal antibody (MAb) production. Two MAbs specific for ICP11 were selected; these MAbs can be used to detect natural WSSV infection in Penaeus vannamei by dot blotting, western blotting or immunohistochemistry without cross-reaction with other shrimp tissues or other common shrimp viruses. The detection sensitivity of the MAbs was approximately 0. 7 fmole/spot of GST-ICP11 as determined by dot blotting. These MAbs showed stronger immunoreactivity than other MAbs from previous studies that are specific for VP28 and VP19. A combination of MAbs specific for ICP11, VP28 and VP19 increased the detection sensitivity of WSSV during early infection to a sensitivity 250 times lower than that of one-step PCR. Therefore, the MAbs specific for ICP11 could be used to confirm and enhance the detection sensitivity for WSSV infection in shrimp using various types of antibody-based assays. © 2012 Springer-Verlag Wien.
URI: https://ir.swu.ac.th/jspui/handle/123456789/14074
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84876719031&doi=10.1007%2fs00705-012-1569-3&partnerID=40&md5=a6e934eb3e6131a33376b3e8394309ab
ISSN: 3048608
Appears in Collections:Scopus 1983-2021

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