Please use this identifier to cite or link to this item: https://ir.swu.ac.th/jspui/handle/123456789/14069
ชื่อเรื่อง: Phyllanthus emblica L. Enhances human umbilical vein endothelial wound healing and sprouting
ผู้แต่ง: Chularojmontri L.
Suwatronnakorn M.
Wattanapitayakul S.K.
Keywords: antioxidant
Emblica officinalis extract
gallic acid
messenger RNA
nitric oxide
phenol derivative
vasculotropin
wound healing promoting agent
angiogenesis
antioxidant activity
article
cell migration
cell proliferation
cell survival
cytotoxicity
Emblica officinalis
fluorescence recovery after photobleaching
freeze drying
gene expression
high performance liquid chromatography
human
human cell
in vitro study
priority journal
umbilical vein endothelial cell
wound closure
wound healing
วันที่เผยแพร่: 2013
บทคัดย่อ: Endothelial dysfunction is the hallmark of impaired wound healing and increased risk of cardiovascular disease. Antioxidants from natural sources decrease oxidative stress and protect against cellular damage caused by reactive oxygen species (ROS). In this study, we examined the antioxidant constituents and capacity of Phyllanthus emblica L. (PE) fruit in freeze-dried power form. The pharmacological properties of PE were investigated using human umbilical vein endothelial cells (HUVECs) in the aspects of endothelial cell proliferation, nitric oxide (NO) production, wound healing, cell migration, in vitro angiogenesis, and VEGF gene expression. The ASC content of PE was 1.574% + 0.046% (w/w) as determined by HPLC and the total phenolic content was 36.1% ± 0.7% gallic acid equivalent when measured by Folin-Ciocalteu assay. The FRAP assay revealed a relatively high antioxidant capacity at 3,643 + 192.5 μmole/mg. PE at 0.1 to 10 μg/mL did not significantly influence endothelial cell proliferation, but at higher concentrations PE decreased cell survival to 62%. PE significantly promoted NO production, endothelial wound closure, endothelial sprouting, and VEGF mRNA expression. Therefore, PE is a candidate for antioxidant supplement that promotes endothelial function and restores wound healing competency. © 2013 Linda Chularojmontri et al.
URI: https://ir.swu.ac.th/jspui/handle/123456789/14069
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84877273108&doi=10.1155%2f2013%2f720728&partnerID=40&md5=b31edcd3e85d8abbc2c0be98484cd1c7
ISSN: 1741427X
Appears in Collections:Scopus 1983-2021

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