Please use this identifier to cite or link to this item: https://ir.swu.ac.th/jspui/handle/123456789/13948
ชื่อเรื่อง: Suppression of human fibrosarcoma cell metastasis by Phyllanthus emblica extract in vitro
ผู้แต่ง: Yahayo W.
Supabphol A.
Supabphol R.
Keywords: gelatinase A
gelatinase B
messenger RNA
MMP2 protein, human
MMP9 protein, human
plant extract
apoptosis
article
cell adhesion
cell motion
cell proliferation
chemistry
drug effect
Emblica officinalis
fibrosarcoma
genetics
human
in vitro study
metabolism
metastasis
phytotherapy
real time polymerase chain reaction
reverse transcription polymerase chain reaction
tumor cell culture
Apoptosis
Cell Adhesion
Cell Movement
Cell Proliferation
Fibrosarcoma
Humans
Matrix Metalloproteinase 2
Matrix Metalloproteinase 9
Phyllanthus emblica
Phytotherapy
Plant Extracts
Real-Time Polymerase Chain Reaction
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger
Tumor Cells, Cultured
วันที่เผยแพร่: 2013
บทคัดย่อ: Phyllanthus emblica (PE) is known to exhibit various pharmacological properties. This study aimed to evaluate the antimetastatic potential of a PE aqueous extract. Cytotoxicity to human fibrosarcoma cells, HT1080, was determined by viability assay using the 3-(4,5-dimethylthiazol,2-yl)-2,5-diphenyltetrazolium bromide (MTT) reagent. Cell migration and invasion were investigated using chemotaxis chambers containing membranes precoated with collagen IV and Matrigel, respectively. Cell attachment onto normal surfaces of cell culture plates was tested to determine the cell-adhesion capability. The molecular mechanism of antimetastatic activity was assessed by measuring the gene expression of matrix metalloproteinases, MMP2, and MMP9, using reverse transcription-polymerase chain reaction (RT-PCR) assay. The mRNA levels of both genes were significantly down-regulated after pretreatment with PE extract for 5 days. Our findings show the antimetastatic function of PE extract in reducing cell proliferation, migration, invasion, and adhesion in both dose- and time-dependent manners, especially growth arrest with low IC50 value. A decrease in the expression of both MMP2 and MMP9 seems to be the cellular mechanism for antimetastasis in this case. There is a high potential to use PE extracts clinically as an optional adjuvant therapeutic drug for therapeutic intervention strategies in cancer therapy or chemoprevention.
URI: https://ir.swu.ac.th/jspui/handle/123456789/13948
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84892456221&doi=10.7314%2fAPJCP.2013.14.11.6863&partnerID=40&md5=2764582b8652f0b48ddb8d9471c54fd1
ISSN: 15137368
Appears in Collections:Scopus 1983-2021

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