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DC Field | Value | Language |
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dc.contributor.author | Chunta S. | |
dc.contributor.author | Suk-Anake J. | |
dc.contributor.author | Chansiri K. | |
dc.contributor.author | Promptmas C. | |
dc.date.accessioned | 2021-04-05T03:32:44Z | - |
dc.date.available | 2021-04-05T03:32:44Z | - |
dc.date.issued | 2014 | |
dc.identifier.issn | 32654 | |
dc.identifier.other | 2-s2.0-84906092923 | |
dc.identifier.uri | https://ir.swu.ac.th/jspui/handle/123456789/13944 | - |
dc.identifier.uri | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84906092923&doi=10.1039%2fc4an00601a&partnerID=40&md5=c94493ca3cc35b31782702fed3a0636c | |
dc.description.abstract | A piezoelectric-based immunosensor was developed for high density lipoprotein particle (HDL-P) measurement. Monoclonal anti-human apolipoprotein A1 antibody was used as a specific binding molecule for the major apolipoprotein of HDL-P. This sensing element was fabricated by immobilizing the anti-human apolipoprotein A1 on a 12 MHz AT-cut quartz crystal via a 3-mercaptopropionic acid (MPA) self-assembled monolayer. The frequency shift from the mass change of the antigen-antibody binding refers to the amount of HDL-P. The optimal antibody immobilization was performed to achieve the maximum potential of the antibody. The appropriate quantity and immobilization time of the antibody were 0.1 mg ml−1 and 90 minutes, respectively. The immobilized antibody in the HDL-P immunosensor accomplished perfect binding with HDL-P within 60 minutes. The dose-response curve for HDL-P showed a linear response from 0.21 to 2.50 mg protein per ml equivalent to 0.40 × 1010 to 3.65 × 1010 particles per μl without significant interference from other lipoproteins. The intra- and inter-assay imprecision (CV) were 7.8 and 18.5%, respectively. The analytical accuracy of this measurement was 96.29-96.31%. The HDL-P concentration obtained from the sensor revealed a 2.05 mg protein per ml with 0.26 mg protein per ml of expanded uncertainty at the 95% confidence level. This immunosensor gave an assay result which correlated with the homogeneous enzymatic colorimetric assay (R2 = 0.902). © 2014 the Partner Organisations. | |
dc.title | A piezoelectric-based immunosensor for high density lipoprotein particle measurement | |
dc.type | Article | |
dc.rights.holder | Scopus | |
dc.identifier.bibliograpycitation | Analyst. Vol 139, No.18 (2014), p.4586-4592 | |
dc.identifier.doi | 10.1039/c4an00601a | |
Appears in Collections: | Scopus 1983-2021 |
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