Please use this identifier to cite or link to this item: https://ir.swu.ac.th/jspui/handle/123456789/13921
ชื่อเรื่อง: Rat β3-adrenoceptor protein expression: Antibody validation and distribution in rat gastrointestinal and urogenital tissues
ผู้แต่ง: Cernecka H.
Pradidarcheep W.
Lamers W.H.
Schmidt M.
Michel M.C.
Keywords: ab 15688
antibody
beta 3 adrenergic receptor
sc 1473
unclassified drug
antibody
beta 3 adrenergic receptor
animal cell
animal tissue
Article
bladder
epithelium cell
gastrointestinal tract
genotype
immunoblotting
immunocytochemistry
immunohistochemistry
mouse
nonhuman
polymerase chain reaction
protein expression
rat
stomach
urogenital system
urothelium
animal
C57BL mouse
CHO cell line
comparative study
Cricetulus
female
gastrointestinal tract
genetics
hamster
immunohistochemistry
immunology
knockout mouse
male
procedures
urogenital system
Wistar rat
Animals
Antibodies
CHO Cells
Cricetinae
Cricetulus
Female
Gastrointestinal Tract
Immunoblotting
Immunohistochemistry
Male
Mice
Mice, Inbred C57BL
Mice, Knockout
Rats
Rats, Wistar
Receptors, Adrenergic, beta-3
Urogenital System
วันที่เผยแพร่: 2014
บทคัดย่อ: β3-Adrenoceptors play important roles in the regulation of urogenital and probably gastrointestinal function. However, despite recent progress, their detection at the protein level has remained difficult due to a lack of sufficiently validated selective antibodies. Therefore, we have explored the selectivity of two antibodies for the detection of rodent β3-adrenoceptors in immunoblots and immunohistochemistry. Of two reportedly promising candidates, antibody AB15688 did not exhibit subtype selectivity in immunoblots. In contrast, the antibody Sc1473 exhibited at least some selectivity in immunoblots and more promising results in immunocytochemical and immunohistochemical stains in cells transfected with cloned β-adrenoceptor subtypes and in rat and mouse tissues. In a systematic screening of rat gastrointestinal and urogenital tissues, Sc1473 produced selective staining in the epithelial cell lining of the stomach and the urothelium of ureter and bladder. We conclude that the two tested antibodies are inappropriate or at least insufficient for immunoblotting applications, but Sc1473 appears to be useful for immunohistochemical detection of β3-adrenoceptor protein in rodent tissues. The β3-adrenoceptor protein exhibits a distinct expression pattern in the rat gastrointestinal and urogenital tract, which is at least partly in line with previously reported functional data. © 2014 Springer-Verlag Berlin Heidelberg.
URI: https://ir.swu.ac.th/jspui/handle/123456789/13921
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84919731329&doi=10.1007%2fs00210-014-1039-4&partnerID=40&md5=b77b05c25ec11bd394fd574635adfc93
ISSN: 281298
Appears in Collections:Scopus 1983-2021

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