Please use this identifier to cite or link to this item: https://ir.swu.ac.th/jspui/handle/123456789/13915
ชื่อเรื่อง: P2X7 receptor-Pannexin1 interaction mediates stress-induced interleukin-1 beta expression in human periodontal ligament cells
ผู้แต่ง: Kanjanamekanant K.
Luckprom P.
Pavasant P.
Keywords: adenosine triphosphate
carbenoxolone
connexin 43
connexin 50
gap junction protein
GJD4 protein, human
interleukin 1beta
meclofenamic acid
nerve protein
PANX1 protein, human
probenecid
purinergic P2X7 receptor
quinine
small interfering RNA
spermine
antagonists and inhibitors
biomechanics
cell culture
culture technique
cytology
genetics
human
immunology
mechanical stress
periodontal ligament
Adenosine Triphosphate
Biomechanical Phenomena
Carbenoxolone
Cell Culture Techniques
Cells, Cultured
Connexin 43
Connexins
Humans
Interleukin-1beta
Meclofenamic Acid
Nerve Tissue Proteins
Periodontal Ligament
Probenecid
Quinine
Receptors, Purinergic P2X7
RNA, Small Interfering
Spermine
Stress, Mechanical
วันที่เผยแพร่: 2014
บทคัดย่อ: Background and Objective: Pannexin 1 (Panx1) has been found to form nonjunctional hemichannels. It is also proposed to combine with the P2X7 receptor, forming a complex involved in adenosine triphosphate (ATP)-induced interleukin-1beta (IL-1β) release in macrophages. Previously, we reported that mechanical stress induced IL-1β expression via the ATP/P2X7 receptor-dependent pathway in human periodontal ligament (HPDL) cells and that ATP was released through the connexin 43 (Cx43) hemichannel. In the present work, we examined the role of Panx1 in stress-induced IL-1β induction in HPDL cells. Material and Methods: Cultured HPDL cells were treated with compressive loading or ATP to stimulate IL-1β expression. Inhibitors, antagonists and the small interfering RNA technique were used to investigate the involvement of Panx1 in IL-1β induction. Co-immunoprecipitation (Co-IP) and immunostaining were used to determine the association of Panx1 with the P2X7 receptor. The IL-1β release mechanism was analyzed using inhibitors. Results: Blocking Panx1 significantly decreased ATP release, as well as IL-1β up-regulation, upon stimulation with stress or ATP. Co-IP revealed the association of Panx1 and the P2X7 receptor in HPDL cells, which was increased in response to mechanical loading. Pretreatment with vesicular trafficking inhibitors significantly reduced the amount of IL-1β released from stimulated cells, suggesting that IL-1β might be released through vesicles. Conclusion: We clearly illustrated the contribution of Panx1 in ATP release, as well as in IL-1β induction in HPDL cells. The association of Panx1 and the P2X7 receptor might be required for IL-1β induction, and their possible novel role in IL-1β vesicular release was indicated. © 2013 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.
URI: https://ir.swu.ac.th/jspui/handle/123456789/13915
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84908596612&doi=10.1111%2fjre.12139&partnerID=40&md5=99b53c57925a819ddb7bd31c08e1eaf9
ISSN: 223484
Appears in Collections:Scopus 1983-2021

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