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DC Field | Value | Language |
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dc.contributor.author | Plaon S. | |
dc.contributor.author | Longyant S. | |
dc.contributor.author | Sithigorngul P. | |
dc.contributor.author | Chaivisuthangkura P. | |
dc.date.accessioned | 2021-04-05T03:26:25Z | - |
dc.date.available | 2021-04-05T03:26:25Z | - |
dc.date.issued | 2015 | |
dc.identifier.issn | 8997659 | |
dc.identifier.other | 2-s2.0-84940045331 | |
dc.identifier.uri | https://ir.swu.ac.th/jspui/handle/123456789/13779 | - |
dc.identifier.uri | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84940045331&doi=10.1080%2f08997659.2015.1037468&partnerID=40&md5=bf42cede0883a05711c8c7de7b78d58e | |
dc.description.abstract | Vibrio alginolyticus is a major bacterial pathogen causing disease in marine animals. The present study aimed to develop a loop-mediated isothermal amplification (LAMP) coupled with a lateral flow dipstick (LFD) for rapid and simple visual detection of V. alginolyticus–specific amplicons. The biotin-labeled LAMP amplicons from the targeted portion of a gene encoding rpoS-like sigma factor (rpoX) were generated at 60°C for 1 h and then hybridized with a fluorescein isothiocyanate–labeled probe for 5 min for visual detection with LFD. In pure cultures, the detection limit of the LAMP–LFD technique for V. alginolyticus was 1.8 × 102 CFU/mL while that of PCR was 1.8 × 103 CFU/mL. In spiked whiteleg shrimp samples Penaeus vannamei, the sensitivity for V. alginolyticus detection was 2 × 103 CFU/g (equivalent to 4 CFU per reaction) while PCR was 10 times less sensitive. The LAMP–LFD method for V. alginolyticus correctly identified 21 isolates of V. alginolyticus but did not recognize 23 non-V. alginolyticus Vibrio isolates and 15 non-Vibrio isolates. In summary, this LAMP–LFD method targeted to the rpoX gene is a convenient assay for specific identification of V. alginolyticus with high sensitivity. © American Fisheries Society 2015. | |
dc.subject | Animalia | |
dc.subject | Bacteria (microorganisms) | |
dc.subject | Litopenaeus vannamei | |
dc.subject | Vibrio | |
dc.subject | Vibrio alginolyticus | |
dc.subject | bacterial protein | |
dc.subject | molecular probe | |
dc.subject | chemistry | |
dc.subject | gene expression regulation | |
dc.subject | genetics | |
dc.subject | isolation and purification | |
dc.subject | metabolism | |
dc.subject | molecular probe | |
dc.subject | nucleic acid amplification | |
dc.subject | physiology | |
dc.subject | procedures | |
dc.subject | Vibrio alginolyticus | |
dc.subject | Bacterial Proteins | |
dc.subject | Gene Expression Regulation, Bacterial | |
dc.subject | Molecular Probes | |
dc.subject | Nucleic Acid Amplification Techniques | |
dc.subject | Vibrio alginolyticus | |
dc.title | Rapid and sensitive detection of Vibrio alginolyticus by loop-mediated isothermal amplification combined with a lateral flow dipstick targeted to the rpoX gene | |
dc.type | Article | |
dc.rights.holder | Scopus | |
dc.identifier.bibliograpycitation | Journal of Aquatic Animal Health. Vol 27, No.3 (2015), p.156-163 | |
dc.identifier.doi | 10.1080/08997659.2015.1037468 | |
Appears in Collections: | Scopus 1983-2021 |
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