Please use this identifier to cite or link to this item: https://ir.swu.ac.th/jspui/handle/123456789/13776
ชื่อเรื่อง: Mechanism of apoptosis induction associated with ERK1/2 upregulation via goniothalamin in melanoma cells
ผู้แต่ง: Tangchirakhaphan S.
Innajak S.
Nilwarangkoon S.
Tanjapatkul N.
Mahabusrakum W.
Watanapokasin R.
Keywords: antineoplastic agent
caspase 7
caspase 9
goniothalamin
mitogen activated protein kinase 1
mitogen activated protein kinase 3
nicotinamide adenine dinucleotide adenosine diphosphate ribosyltransferase 1
protein bcl 2
protein bcl xl
protein mcl 1
unclassified drug
analysis of variance
antiinflammatory activity
antimicrobial activity
antineoplastic activity
apoptosis
Article
cell cycle assay
cell growth
cell viability assay
chromatin condensation
controlled study
cytotoxicity
fluorescence microscopy
human
human cell
IC50
melanoma
mitochondrial membrane
mitochondrial membrane potential
morphological adaptation
MTT assay
protein phosphorylation
receptor upregulation
วันที่เผยแพร่: 2018
บทคัดย่อ: The present study aimed to investigate the effect of goniothalamin on apoptosis induction in the A375 melanoma cell line. Melanoma is a type of skin cancer with increased prevalence and no potential standard treatment. Goniothalamin is a plant, bioactive styrly-lactone, which has various bioactivities including anti-microbial, anti-inflammatory and anti-cancer. Apoptosis induction by goniothalamin has been studied in numerous cancer cell lines, however not in the melanoma cell line A375. The results of the MTT assay demonstrated that goniothalamin induced anti-proliferation in a dose dependent manner. Hoechst staining assay demonstrated that goniothalamin induced chromatin condensation and apoptotic bodies in A375 treated cells, and JC-1 staining revealed that goniothalamin induced mitochondrial membrane dysfunction in A375 cells. In addition, goniothalamin decreased the level of anti-apoptotic proteins myeloid cell leukemia 1, B cell lymphoma (Bcl)-2 and Bcl-extra large, whereas it increased the level of pro-apoptotic proteins, Bcl-2 Associated X, apoptosis regulator, t-BID and Bim in A375 treated cells. In addition, goniothalamin also increased active caspase-9, -7 and cleaved-poly (ADP-ribose) polymerase expression in A375 treated cells. Furthermore, phosphorylated (p)-pyruvate dehydrogenase kinase (PDK) 1 (Ser241) and p-RAC-alpha serine/threonine-protein kinase (Akt; Ser473) were decreased, however c-Jun and p-extracellular signal-regulated kinase (ERK)1/2 were increased upon goniothalamin treatment. These results suggest that goniothalamin has an effect, as anti-proliferation and apoptosis induction in A375 cells were associated with upregulated p-ERK1/2, c-Jun and downregulated p-PDK1 (Ser241), p-Akt (Ser473) in A375 cells. Therefore, goniothalamin may be a potential candidate for anti-cancer drug development for melanoma treatment. © 2018, Spandidos Publications. All rights reserved.
URI: https://ir.swu.ac.th/jspui/handle/123456789/13776
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85041199338&doi=10.3892%2fetm.2018.5762&partnerID=40&md5=6708e81104243382fcc1ee0a4d5db026
ISSN: 17920981
Appears in Collections:Scopus 1983-2021

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