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DC Field | Value | Language |
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dc.contributor.author | Thongkao K. | |
dc.contributor.author | Longyant S. | |
dc.contributor.author | Silprasit K. | |
dc.contributor.author | Sithigorngul P. | |
dc.contributor.author | Chaivisuthangkura P. | |
dc.date.accessioned | 2021-04-05T03:25:48Z | - |
dc.date.available | 2021-04-05T03:25:48Z | - |
dc.date.issued | 2015 | |
dc.identifier.issn | 1355557X | |
dc.identifier.other | 2-s2.0-84926418226 | |
dc.identifier.uri | https://ir.swu.ac.th/jspui/handle/123456789/13698 | - |
dc.identifier.uri | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84926418226&doi=10.1111%2fare.12266&partnerID=40&md5=f4ae4a5f1624a70c589c5f92b1dd2379 | |
dc.description.abstract | Vibrio harveyi is a causative agent of the Vibriosis or luminescent bacterial disease in worldwide aquaculture industry. A reliable assay for identification of V. harveyi infection is important to prevent the bacterial spread. In this study, biotinylated loop-mediated isothermal amplification (LAMP) amplicons were produced by a set of four designed primers that recognized specifically the V. harveyi vhhP2 gene, encoding a putative outer membrane protein with unknown function, followed by hybridization with an fluorescein isothiocyanate (FITC)-labelled probe and lateral flow dipstick (LFD) detection. A novel set of PCR primer was also designed specifically to vhhP2 gene and appear to be a species-specific tool for V. harveyi detection. The optimized time and temperature conditions for the LAMP assay were 90 min at 65°C. The LAMP-LFD and PCR methods accurately identified 22 isolates of V. harveyi but did not detect 16 non-harveyi Vibrio isolates, and 34 non-Vibrio bacterial isolates. The sensitivity of LAMP-LFD for V. harveyi detection in pure culture was 1.1 × 102 CFU mL-1 or equivalent to 0.6 CFU per reaction, while that of PCR was 6 CFU per reaction. For spiked shrimp sample, the sensitivity of LAMP was 1.8 × 103 CFU g-1 or equivalent to 5 CFU per reaction, while that of PCR was 50 CFU per reaction. In conclusion, the established LAMP-LFD methods provided a valuable tool for rapid identification of V. harveyi and can be used to distinguish V. harveyi from V. campbellii. © 2013 John Wiley & Sons Ltd. | |
dc.subject | aquaculture industry | |
dc.subject | bacterium | |
dc.subject | bioassay | |
dc.subject | gene | |
dc.subject | gene expression | |
dc.subject | hybridization | |
dc.subject | isotherm | |
dc.subject | polymerase chain reaction | |
dc.subject | sensitivity analysis | |
dc.subject | temperature effect | |
dc.subject | Bacteria (microorganisms) | |
dc.subject | Decapoda (Crustacea) | |
dc.subject | Vibrio | |
dc.subject | Vibrio campbellii | |
dc.subject | Vibrio harveyi | |
dc.title | Rapid and sensitive detection of Vibrio harveyi by loop-mediated isothermal amplification combined with lateral flow dipstick targeted to vhhP2 gene | |
dc.type | Article | |
dc.rights.holder | Scopus | |
dc.identifier.bibliograpycitation | Aquaculture Research. Vol 46, No.5 (2015), p.1122-1131 | |
dc.identifier.doi | 10.1111/are.12266 | |
Appears in Collections: | Scopus 1983-2021 |
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