Please use this identifier to cite or link to this item: https://ir.swu.ac.th/jspui/handle/123456789/13696
ชื่อเรื่อง: In vitro modulation of tumor necrosis factor α production in THP-1 cells by lactic acid bacteria isolated from healthy human infants
ผู้แต่ง: Ladda B.
Theparee T.
Chimchang J.
Tanasupawat S.
Taweechotipatr M.
Keywords: DNA 16S
probiotic agent
tumor necrosis factor alpha
probiotic agent
tumor necrosis factor
acid tolerance
Article
bacterial strain
bacterium culture
bacterium isolate
cell free system
cytokine production
DNA sequence
embryo
feces analysis
heating
human
immunomodulation
Klebsiella pneumoniae
lactic acid bacterium
Lactobacillus casei
Lactobacillus paracasei
monocyte
nonhuman
priority journal
Weissella
Weissella confusa
adaptation
biosynthesis
cell culture
cell line
feces
immunology
immunomodulation
infant
intestine mucosa
isolation and purification
Lactobacillus
metabolism
microbiology
newborn
physiology
Lactobacillus casei
Lactobacillus paracasei
Weissella confusa
Adaptation, Physiological
Cell Line
Cells, Cultured
Feces
Humans
Immunomodulation
Infant
Infant, Newborn
Intestinal Mucosa
Lactobacillus
Monocytes
Probiotics
Tumor Necrosis Factor-alpha
วันที่เผยแพร่: 2015
บทคัดย่อ: The human microbiota is a source of probiotics capable of modulating the host immune system. In this study, we collected fecal samples from 100 healthy infants and isolated lactic acid bacteria which were screened for immune modulating effects on tumor necrosis factor α (TNF-α) production. Cell-free culture supernatants from 26 isolates were able to decrease TNF-α production invitro and three of the isolates were selected as candidate probiotics (MSMC39-1, MSMC39-3, MSMC57-1). These isolates were identified using 16S ribosomal DNA sequencing as Lactobacillus paracasei, Lactobacillus casei, and Weissella confusa respectively. All three isolates were acid tolerant and bile tolerant to pH 3.0 and 4% bile respectively. Preparations of cell-free culture supernatants were processed and tested, and revealed that cell-free culture supernatants of isolates L.paracasei MSMC39-1, L.casei MSMC39-3, and W.confusa MSMC57-1 decreased the production of TNF-α significantly and were heat resistant. Only L.paracasei MSMC39-1 supernatant was proteinase-K sensitive. The effects of viable bacteria, heat-killed bacteria, and sonicated bacteria were compared. The heat-killed preparations of isolate W.confusa MSMC57-1 decreased the production of TNF-α. Sonicated cell preparations did not significantly alter TNF-α production. For isolates L.paracasei MSMC39-1 and L.casei MSMC39-3, this suggests that a substance in the cell-free culture supernatant may be responsible for invitro cytokine modulation. © 2015 Elsevier Ltd.
URI: https://ir.swu.ac.th/jspui/handle/123456789/13696
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84924532766&doi=10.1016%2fj.anaerobe.2015.03.002&partnerID=40&md5=a7f2525a8a42b20adb12db3aea1c4242
ISSN: 10759964
Appears in Collections:Scopus 1983-2021

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