Please use this identifier to cite or link to this item: https://ir.swu.ac.th/jspui/handle/123456789/13685
ชื่อเรื่อง: ESI-MS investigation of an equilibrium between a bimolecular quadruplex DNA and a duplex DNA/RNA hybrid
ผู้แต่ง: Birrento M.L.
Bryan T.M.
Samosorn S.
Beck J.L.
Keywords: Bins
Circular dichroism spectroscopy
Complexation
Dichroism
DNA
Electrodeposition
Electrospray ionization
Ionization
Ligands
Mass spectrometry
Melting
Oncogenic viruses
RNA
Spectrometers
Thermodynamic stability
Transcription
DNA secondary structures
Electrospray ionization mass spectrometry
Hybrid structure
Melting profiles
Quadruplex dnas
Simultaneous observation
Telomerase
Transcriptional control
Nucleic acids
berberine derivative
DNA
double stranded DNA
guanine quadruplex
RNA
telomerase
Article
circular dichroism
DNA RNA hybridization
DNA sequence
DNA synthesis
electrospray mass spectrometry
protein secondary structure
telomere
thermostability
chemistry
electrospray mass spectrometry
procedures
DNA
G-Quadruplexes
RNA
Spectrometry, Mass, Electrospray Ionization
Telomerase
วันที่เผยแพร่: 2015
บทคัดย่อ: Electrospray ionization mass spectrometry (ESI-MS) conditions were optimized for simultaneous observation of a bimolecular qDNA and a Watson-Crick base-paired duplex DNA/RNA hybrid. The DNA sequence used was telomeric DNA, and the RNA contained the template for telomerase-mediated telomeric DNA synthesis. Addition of RNA to the quadruplex DNA (qDNA) resulted in formation of the duplex DNA/RNA hybrid. Melting profiles obtained using circular dichroism spectroscopy confirmed that the DNA/RNA hybrid exhibited greater thermal stability than the bimolecular qDNA in solution. Binding of a 13-substituted berberine (1) derivative to the bimolecular qDNA stabilized its structure as evidenced by an increase in its stability in the mass spectrometer, and an increase in its circular dichroism (CD) melting temperature of 10°C. The DNA/RNA hybrid did not bind the ligand extensively and its thermal stability was unchanged in the presence of (1). The qDNA-ligand complex resisted unfolding in the presence of excess RNA, limiting the formation of the DNA/RNA hybrid. Previously, it has been proposed that DNA secondary structures, such as qDNA, may be involved in the telomerase mechanism. DNA/RNA hybrid structures occur at the active site of telomerase. The results presented in the current work show that if telomeric DNA was folded into a qDNA structure, it is possible for a DNA/RNA hybrid to form as is required during template alignment. The discrimination of ligand (1) for binding to the bimolecular qDNA over the DNA/RNA hybrid positions it as a useful compound for probing the role(s), if any, of antiparallel qDNA in the telomerase mechanism. [Figure not available: see fulltext.] © 2015 American Society for Mass Spectrometry.
URI: https://ir.swu.ac.th/jspui/handle/123456789/13685
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84934931455&doi=10.1007%2fs13361-015-1121-2&partnerID=40&md5=c184948a2add7c3984c83fff7f1d39d7
ISSN: 10440305
Appears in Collections:Scopus 1983-2021

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