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https://ir.swu.ac.th/jspui/handle/123456789/13669
ชื่อเรื่อง: | A novel paper-based device coupled with a silver nanoparticle-modified boron-doped diamond electrode for cholesterol detection |
ผู้แต่ง: | Nantaphol S. Chailapakul O. Siangproh W. |
Keywords: | Analytic equipment Chemical detection Cholesterol Diamonds Electrodes Hydrophilicity Metal nanoparticles Nanoparticles Paper Screen printing Silver Amperometric detection Boron doped diamond electrodes ELectrochemical detection Electrodeposition methods Hydrophilic and hydrophobic Paper based devices Paper-based analytical devices Silver nanoparticles Electrochemical electrodes boron cholesterol cholesterol oxidase diamond hydrogen peroxide silver nanoparticle boron cholesterol diamond metal nanoparticle silver adult amperometry analytical equipment Article biosensor bovine chemical modification chemical phenomena cholesterol blood level controlled study cost control electrochemical detection electrode electrodeposition filter paper hydrophilicity hydrophobicity limit of detection lipid analysis measurement precision nanofabrication nonhuman paper based analytical device portable equipment printing priority journal reaction time screen printing sensitivity analysis animal blood chemistry devices electrochemical analysis electrode equipment design evaluation study genetic procedures paper Animals Biosensing Techniques Boron Cattle Cholesterol Diamond Electrochemical Techniques Electrodes Equipment Design Limit of Detection Metal Nanoparticles Paper Silver |
วันที่เผยแพร่: | 2015 |
บทคัดย่อ: | A novel paper-based analytical device (PAD) coupled with a silver nanoparticle-modified boron-doped diamond (AgNP/BDD) electrode was first developed as a cholesterol sensor. The AgNP/BDD electrode was used as working electrode after modification by AgNPs using an electrodeposition method. Wax printing was used to define the hydrophilic and hydrophobic areas on filter paper, and then counter and reference electrodes were fabricated on the hydrophilic area by screen-printing in house. For the amperometric detection, cholesterol and cholesterol oxidase (ChOx) were directly drop-cast onto the hydrophilic area, and H2O2 produced from the enzymatic reaction was monitored. The fabricated device demonstrated a good linearity (0.39 mg dL-1 to 270.69 mg dL-1), low detection limit (0.25 mg dL-1), and high sensitivity (49.61 μA mM-1 cm-2). The precision value for ten replicates was 3.76% RSD for 1 mM H2O2. In addition, this biosensor exhibited very high selectivity for cholesterol detection and excellent recoveries for bovine serum analysis (in the range of 99.6-100.8%). The results showed that this new sensing platform will be an alternative tool for cholesterol detection in routine diagnosis and offers the advantages of low sample/reagent consumption, low cost, portability, and short analysis time. © 2015 Elsevier B.V. |
URI: | https://ir.swu.ac.th/jspui/handle/123456789/13669 https://www.scopus.com/inward/record.uri?eid=2-s2.0-84941874160&doi=10.1016%2fj.aca.2015.08.007&partnerID=40&md5=7849373478273ee7e8f0c829b121d56b |
ISSN: | 32670 |
Appears in Collections: | Scopus 1983-2021 |
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