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ชื่อเรื่อง: | Antioxidant, aα-glucosidase inhibitory activity and sub-chronic toxicity of Derris reticulata extract: Its antidiabetic potential |
ผู้แต่ง: | Kumkrai P. Weeranantanapan O. Chudapongse N. |
Keywords: | 1,1 diphenyl 2 picrylhydrazyl 2,2' azinobis(3 ethylbenzothiazoline 6 sulfonic acid) acarbose alloxan alpha glucosidase anthraquinone derivative antidiabetic agent antioxidant ascorbic acid cardiac glycoside Derris reticulata extract flavanoid flavonoid glibenclamide insulin phenol derivative plant extract saponin derivative tannin derivative terpenoid derivative unclassified drug water acarbose alpha glucosidase antidiabetic agent antineoplastic agent antioxidant flavonoid glibenclamide glucose blood level glycosidase inhibitor insulin phenol derivative plant extract tannin derivative alloxan-induced diabetes mellitus animal cell animal experiment animal model animal tissue antidiabetic activity antioxidant activity Article biochemistry blood toxicity cell damage cell viability chemical composition chronic toxicity controlled study Derris Derris reticulata drug fatality drug isolation drug screening enzyme activity enzyme inhibition female fluorescence recovery after photobleaching histopathology in vitro study insulin release male nonhuman oxidative stress phytochemistry rat RINm5f cell line adverse effects animal blood chemistry Diabetes Mellitus, Experimental drug effects glucose blood level metabolism pancreas islet beta cell phytotherapy Wistar rat Acarbose Alloxan alpha-Glucosidases Animals Antineoplastic Agents, Phytogenic Antioxidants Blood Glucose Derris Diabetes Mellitus, Experimental Female Flavonoids Glyburide Glycoside Hydrolase Inhibitors Hypoglycemic Agents Insulin Insulin-Secreting Cells Male Phenols Phytotherapy Plant Extracts Rats, Wistar Tannins |
วันที่เผยแพร่: | 2015 |
บทคัดย่อ: | Background: Antidiabetic activity of Derris reticulata extract on alloxan-induced diabetic rats has been reported. The extract was found to lower blood glucose and inhibit intestinal glucose absorption. The aim of this study was to further investigate mechanisms underlying the antihyperglycemic activity of D. reticulata extract in vitro. Methods: The aqueous extract was obtained from D. reticulata stem. Phytochemical screening, total phenolic, and flavanoid contents were examined. ABTS and DPPH scavenging assays, and FRAP method were used to determine in vitro antioxidant activities. Measurement of cell viability on alloxan-induced cellular damage was performed in the insulin-secreting RINm5F cells by MTT assay. The effects of the extract on aα-glucosidase activity and insulin release were studied. In addition, sub-chronic toxicity test in rats was also conducted. Results: The results revealed that the extract, which consisted of terpenoids, saponins, tannins and flavonoids, possessed moderate radical scavenging activities. Pre-treatment of RINm5F cells with the extract was also found to exert moderate, but significant, in vitro protection against alloxan, an oxidative stress producing agent. Unlike glibenclamide, the extract did not stimulate insulin secretion. However, the extract was found to inhibit aα-glucosidase activity similar to acarbose. It was found that in sub-chronic toxicity studies D. reticulata extract did not cause mortality or produce any remarkable haematological, biochemical and histopathological adverse effects in rats. Conclusions: The data suggest that the possible mechanisms underlying antihyperglycemic activity of D. reticulata extract are cytoprotective effect on pancreatic cells, presumably by its antioxidant activity, and inhibition of aα-glucosidase. Sub-chronic toxicity study also provides scientific evidence to corroborate the safety of this plant as an alternative antidiabetic agent. © 2015 Kumkrai et al.; licensee BioMed Central. |
URI: | https://ir.swu.ac.th/jspui/handle/123456789/13596 https://www.scopus.com/inward/record.uri?eid=2-s2.0-84928718716&doi=10.1186%2fs12906-015-0552-4&partnerID=40&md5=5f7a49b833f850bd6462ba6b255cad74 |
ISSN: | 14726882 |
Appears in Collections: | Scopus 1983-2021 |
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