Please use this identifier to cite or link to this item: https://ir.swu.ac.th/jspui/handle/123456789/13580
ชื่อเรื่อง: Engineering of a highly efficient Escherichia coli strain for mevalonate fermentation through chromosomal integration
ผู้แต่ง: Wang J.
Niyompanich S.
Tai Y.-S.
Wang J.
Bai W.
Mahida P.
Gao T.
Zhang K.
Keywords: Bottles
Escherichia coli
Genes
Integration
Metabolism
Productivity
Chromosomal integration
Constitutive promoters
Fed-batch fermentation
Genetic instability
Maximum productivity
Metabolic pathways
Mevalonate pathway
Shake flask fermentations
Fermentation
bioengineering
chromosome
fecal coliform
fermentation
gene expression
glucose
metabolism
plasmid
polymer
Escherichia coli
glucose
mevalonic acid
bacterial chromosome
Escherichia coli
fermentation
genetics
metabolic engineering
metabolism
plasmid
Chromosomes, Bacterial
Escherichia coli
Fermentation
Glucose
Metabolic Engineering
Mevalonic Acid
Plasmids
วันที่เผยแพร่: 2016
บทคัดย่อ: Chromosomal integration of heterologous metabolic pathways is optimal for industrially relevant fermentation, as plasmidbased fermentation causes extra metabolic burden and genetic instabilities. In this work, chromosomal integration was adapted for the production of mevalonate, which can be readily converted into β-methyl-δ-valerolactone, a monomer for the production of mechanically tunable polyesters. The mevalonate pathway, driven by a constitutive promoter, was integrated into the chromosome of Escherichia coli to replace the native fermentation gene adhE or ldhA. The engineered strains (CMEV-1 and CMEV-2) did not require inducer or antibiotic and showed slightly higher maximal productivities (0.38 to~0.43 g/liter/h) and yields (67.8 to~71.4% of the maximum theoretical yield) than those of the plasmid-based fermentation. Since the glycolysis pathway is the first module for mevalonate synthesis, atpFH deletion was employed to improve the glycolytic rate and the production rate of mevalonate. Shake flask fermentation results showed that the deletion of atpFH in CMEV-1 resulted in a 2.1- fold increase in the maximum productivity. Furthermore, enhancement of the downstream pathway by integrating two copies of the mevalonate pathway genes into the chromosome further improved the mevalonate yield. Finally, our fedbatch fermentation showed that, with deletion of the atpFH and sucA genes and integration of two copies of the mevalonate pathway genes into the chromosome, the engineered strain CMEV-7 exhibited both high maximal productivity (~1.01 g/liter/h) and high yield (86.1% of the maximum theoretical yield, 30 g/liter mevalonate from 61 g/liter glucose after 48 h in a shake flask). © 2016, American Society for Microbiology. All Rights Reserved.
URI: https://ir.swu.ac.th/jspui/handle/123456789/13580
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84997173327&doi=10.1128%2fAEM.02178-16&partnerID=40&md5=b951855ffd7b58198ce5e5ce4efb3204
ISSN: 992240
Appears in Collections:Scopus 1983-2021

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