Please use this identifier to cite or link to this item: https://ir.swu.ac.th/jspui/handle/123456789/13411
ชื่อเรื่อง: Cholesterol-based cationic liposome increases dsRNA protection of yellow head virus infection in Penaeus vannamei
ผู้แต่ง: Sanitt P.
Apiratikul N.
Niyomtham N.
Yingyongnarongkul B.-E.
Assavalapsakul W.
Panyim S.
Udomkit A.
Keywords: Cholesterol
Complexation
Efficiency
Genes
Liposomes
RNA
Viruses
Cationic liposomes
Continuous feeding
Dose-dependent manner
Double-stranded RNA
Enzymatic digestions
Protection efficiency
RNA interference
Shrimp
Shellfish
cholesterol
double stranded RNA
liposome
Rab7 protein
cholesterol
double stranded RNA
liposome
animal experiment
animal tissue
antiviral activity
Article
controlled study
diet
dose response
drug synthesis
gene delivery system
genetic transfection
infection prevention
mortality
nanoencapsulation
nonhuman
Penaeus
Penaeus vannamei
priority journal
shrimp
single-stranded RNA virus
virus infection
virus inhibition
yellow head virus
yellow head virus infection
animal
chemistry
drug effects
genetics
metabolism
Nidovirales
Nidovirales Infections
Penaeidae
RNA interference
veterinary
virology
virus replication
Animals
Cholesterol
Liposomes
Nidovirales Infections
Penaeidae
RNA Interference
RNA, Double-Stranded
Roniviridae
Virus Replication
วันที่เผยแพร่: 2016
บทคัดย่อ: Protection of shrimp from yellow head virus (YHV) infection has been demonstrated by injection and oral delivery of dsRNA-YHV protease gene (dsYHV) or shrimp endogenous gene (dsRab7). However, to achieve complete viral suppression and to prolong dsRNA activity, the development of an effective dsRNA delivery system is required. In this study, four cationic liposomes were synthesized and tested for their ability to increase dsRNA efficiency. The results demonstrated that entrapping dsYHV in a cholesterol-based cationic liposome gave the best protection against YHV infection when compared with other cationic lipids. The cholesterol-based cationic liposome-dsYHV (Chol-dsYHV) complex conferred YHV protection in a dose-dependent manner. Injection with Chol-dsYHV at 0.05 μg dsYHV/g shrimp could give comparable level of YHV protection to the injection with 1.25 μg naked dsYHV/g shrimp. The shrimp injected with Chol- dsYHV at 1.25 μg dsRNA/g shrimp showed only 50% mortality at 60 days post injection whereas the naked dsYHV at the same concentration gave 90% mortality. Thus, the liposome-entrapped dsYHV could lower an effective dsRNA concentration in viral protection and prolong dsRNA activity. In addition, encapsulating dsRab7 in the cholesterol-based cationic liposome could protect the dsRab7 from enzymatic digestion, and continuous feeding the shrimp with the diet formulated with the liposome-entrapped dsRab7 for 4 days in the total of 960 μg dsRab7/g shrimp could enhance YHV protection efficiency compared with the naked dsRab7. Our studies reveal that cholesterol-based cationic liposome is a promising dsRNA carrier to enhance dsRNA efficiency in both injection and oral delivery systems. © 2016 Elsevier B.V.
URI: https://ir.swu.ac.th/jspui/handle/123456789/13411
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84966710072&doi=10.1016%2fj.jbiotec.2016.04.049&partnerID=40&md5=b28a9d8b7ebcaba68b14cdc3a6726269
ISSN: 1681656
Appears in Collections:Scopus 1983-2021

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