Please use this identifier to cite or link to this item: https://ir.swu.ac.th/jspui/handle/123456789/13294
ชื่อเรื่อง: Soy isoflavones enhance β-defensin synthesis and secretion in endometrial epithelial cells with exposure to TLR3 agonist polyinosinic-polycytidylic acid
ผู้แต่ง: Srisomboon Y.
Poonyachoti S.
Deachapunya C.
Keywords: beta defensin
beta defensin 1
beta defensin 2
beta defensin 3
beta defensin 4
daidzein
genistein
messenger RNA
polyinosinic polycytidylic acid
antiinfective agent
beta defensin
daidzein
genistein
isoflavone derivative
polyinosinic polycytidylic acid
animal cell
Article
controlled study
drug effect
drug response
endometrium cell
enzyme linked immunosorbent assay
female
gene expression
inoculation
nonhuman
pig
primary cell culture
priority journal
protein secretion
protein synthesis
quantitative analysis
real time polymerase chain reaction
upregulation
animal
cell culture
cytology
endometrium
epithelium cell
gene expression regulation
genetics
immunology
innate immunity
metabolism
soybean
Animals
Anti-Bacterial Agents
beta-Defensins
Cells, Cultured
Endometrium
Epithelial Cells
Female
Gene Expression Regulation
Genistein
Immunity, Innate
Isoflavones
Poly I-C
Soybeans
Swine
วันที่เผยแพร่: 2017
บทคัดย่อ: Problem: β-defensins are important innate chemical barriers that protect the endometrium from pathogen invasion. The effects of soy isoflavones, genistein and daidzein, on the expression and secretion of porcine β-defensins (PBD) in endometrial epithelial cells were investigated under normal or poly I:C-stimulated conditions. Method of study: Primary cultured porcine endometrial epithelial (PE) cells were pretreated with genistein or daidzein followed by poly I:C inoculation. During treatment, the culture media were analyzed for PBD 1-4 secretion by ELISA and the total RNA for PBD gene expression by quantitative RT-PCR. Results: Porcine endometrial epithelial cells constitutively expressed PBD 1-4 and secreted PBD-1, PBD-2, and PBD-4. Genistein and daidzein enhanced PBD-2 expression and PBD-2 and PBD-3 secretion. These compounds also potentiated PBD-2 and PBD-3 expression and secretion which were upregulated by poly I:C. Conclusion: Soy isoflavones, genistein and daidzein, could be potentially used for promoting the innate host defense of endometrium against infection. © 2017 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd
URI: https://ir.swu.ac.th/jspui/handle/123456789/13294
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85018643426&doi=10.1111%2faji.12694&partnerID=40&md5=a23dacb15a155ec8658f7667e2b2e2b6
ISSN: 10467408
Appears in Collections:Scopus 1983-2021

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