Please use this identifier to cite or link to this item: https://ir.swu.ac.th/jspui/handle/123456789/13286
ชื่อเรื่อง: Molecular confirmation of trematodes in the snail intermediate hosts from Ratchaburi Province, Thailand
ผู้แต่ง: Anucherngchai S.
Tejangkura T.
Chontananarth T.
Keywords: internal transcribed spacer 2
Angiostrongylus cantonensis
Article
bootstrapping
cercaria
cercarial infection
conformation
controlled study
DNA extraction
furcocercous cercaria
gene amplification
gene construct
gene sequence
Haplorchis pumilio
Haplorchis taichui
ITS2 gene
Lecithodendriidae
megarulous cercaria
Melanoides tuberculata
nonhuman
parapleurolophocercous cercaria
Philophthalmus
phylogenetic tree
polymerase chain reaction
prevalence
priority journal
sequence alignment
snail
species identification
Tarebia granifera
Thailand
transversotrema cercaria
trematodiasis
xiphidiocercaria
วันที่เผยแพร่: 2017
บทคัดย่อ: Objective: To analyze the prevalence of trematode infections in the snails intermediate host Tarebia granifera and Melanoides tuberculata collected from Ratchaburi Province by a molecular approach in order to construct a dendrogram to identify species at the cercarial stage. Methods: Specimens were collected from nine districts located in Ratchaburi Province using the stratified sampling method. The samples were examined for cercarial infection using the crushing method. All specimens were amplified by region of internal transcribed spacer 2 (ITS2) based on the PCR technique, and the sequence data were aligned in order to construct the dendrogram by maximum likelihood with 10 000 bootstrap replicates. Results: Five cercarial types were observed comprising parapleurolophocercous cercaria, xiphidiocercaria, megarulous cercaria, furcocercous cercaria and transversotrema cercaria. The overall prevalence of cercarial infection was found to be 7.92% (63 / 795). The parapleurolophocercous cercaria was found in the highest level of prevalence followed by xiphidiocercaria, megarulous cercaria, furcocercous cercaria and transversotrema cercaria, respectively. The parapleurolophocercous cercaria samples were identified and separated into Haplorchis taichui and Haplorchis pumilio, while xiphidiocercaria were identified as Lecithodendriidae and megarulous cercaria were identified as Philophthalmus. The dendrogram separated the cercariae into five groups, which were comprised of parapleurolophocercous cercaria, xiphidiocercaria, megarulous cercaria, furcocercous cercaria and transversotrema cercaria using the sequence of Angiostrongylus cantonensis as an out-group. Conclusions: This is the first study to report on the prevalence of cercarial infection in Ratchaburi Province, Thailand. The high prevalence of cercariae was revealed by the infection in Tarebia granifera and Melanoides tuberculata. Thus, the sequence data of ITS2 can be used to investigate the dendrogram of trematodes and can help to separate the collected samples at the species level. This information can be used to create the prevention program for parasite infection from intermediate hosts in the future. © 2017 by the Asian Pacific Journal of Tropical Disease.
URI: https://ir.swu.ac.th/jspui/handle/123456789/13286
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85019603180&doi=10.12980%2fapjtd.7.2017D6-399&partnerID=40&md5=eb57f5d46c2a0adbd02cdd6e896f41a2
ISSN: 22221808
Appears in Collections:Scopus 1983-2021

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