Please use this identifier to cite or link to this item: https://ir.swu.ac.th/jspui/handle/123456789/13158
ชื่อเรื่อง: Electrochemical paper-based peptide nucleic acid biosensor for detecting human papillomavirus
ผู้แต่ง: Teengam P.
Siangproh W.
Tuantranont A.
Henry C.S.
Vilaivan T.
Chailapakul O.
Keywords: Biosensors
Cell culture
Chemical detection
DNA
Electrochemical impedance spectroscopy
Graphene
Ink jet printing
Ketones
Nucleic acids
Oligonucleotides
Peptides
Polyaniline
Polymerase chain reaction
Probes
Voltammetry
acpcPNA
Electrochemical biosensor
ELectrochemical detection
Electrochemical DNA biosensors
Electrochemical signals
Electrostatic attractions
Human papillomavirus
Square wave voltammetry
Electrochemical electrodes
anthraquinone
graphene
nucleic acid
polyaniline
pyrrolidine derivative
pyrrolidinyl peptide nucleic acid
unclassified drug
peptide nucleic acid
virus DNA
amino terminal sequence
Article
biosensor
controlled study
electrochemical detection
electrochemical impedance spectroscopy
electron transport
Human papillomavirus type 16
hybridization
limit of detection
limit of quantitation
nonhuman
paper based electrochemical biosensor
polymerase chain reaction
potentiometry
priority journal
reproducibility
square wave voltammetry
static electricity
uterine cervix cancer
virus detection
chemistry
electrochemical analysis
electrode
genetic procedures
human
Human papillomavirus type 16
isolation and purification
nucleic acid hybridization
tumor cell line
Electrodes
Ink Jet Printing
Nucleic Acids
Peptides
Biosensing Techniques
Cell Line, Tumor
DNA, Viral
Electrochemical Techniques
Electrodes
Human papillomavirus 16
Humans
Nucleic Acid Hybridization
Peptide Nucleic Acids
วันที่เผยแพร่: 2017
บทคัดย่อ: A novel paper-based electrochemical biosensor was developed using an anthraquinone-labeled pyrrolidinyl peptide nucleic acid (acpcPNA) probe (AQ-PNA) and graphene-polyaniline (G-PANI) modified electrode to detect human papillomavirus (HPV). An inkjet printing technique was employed to prepare the paper-based G-PANI-modified working electrode. The AQ-PNA probe baring a negatively charged amino acid at the N-terminus was immobilized onto the electrode surface through electrostatic attraction. Electrochemical impedance spectroscopy (EIS) was used to verify the AQ-PNA immobilization. The paper-based electrochemical DNA biosensor was used to detect a synthetic 14-base oligonucleotide target with a sequence corresponding to human papillomavirus (HPV) type 16 DNA by measuring the electrochemical signal response of the AQ label using square-wave voltammetry before and after hybridization. It was determined that the current signal significantly decreased after the addition of target DNA. This phenomenon is explained by the rigidity of PNA-DNA duplexes, which obstructs the accessibility of electron transfer from the AQ label to the electrode surface. Under optimal conditions, the detection limit of HPV type 16 DNA was found to be 2.3 nM with a linear range of 10–200 nM. The performance of this biosensor on real DNA samples was tested with the detection of PCR-amplified DNA samples from the SiHa cell line. The new method employs an inexpensive and disposable device, which easily incinerated after use and is promising for the screening and monitoring of the amount of HPV-DNA type 16 to identify the primary stages of cervical cancer. © 2016 Elsevier B.V.
URI: https://ir.swu.ac.th/jspui/handle/123456789/13158
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85006381853&doi=10.1016%2fj.aca.2016.11.071&partnerID=40&md5=4887fe3094bb3cda5164e64c3f5e950a
ISSN: 32670
Appears in Collections:Scopus 1983-2021

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