Please use this identifier to cite or link to this item: https://ir.swu.ac.th/jspui/handle/123456789/13136
ชื่อเรื่อง: Purification, characterization, and overexpression of an endo-1,4-β-mannanase from thermotolerant Bacillus sp. SWU60
ผู้แต่ง: Seesom W.
Thongket P.
Yamamoto T.
Takenaka S.
Sakamoto T.
Sukhumsirichart W.
Keywords: Amino acids
Bacteriology
Cloning
Enzymes
Escherichia coli
Filtration
Gene encoding
Genes
Hydrophobic chromatography
Ion exchange
Liquid chromatography
Purification
Bacillus sp
Galactomannans
Glucomannan
Mannan
Recombinant enzymes
Enzyme activity
beta mannosidase
galactomannan
mannan
Bacillus
biosynthesis
chemistry
enzyme activation
enzyme specificity
enzyme stability
enzymology
Escherichia coli
genetics
ion exchange chromatography
isolation and purification
metabolism
nucleotide sequence
procedures
Bacillus
Base Sequence
beta-Mannosidase
Chromatography, Ion Exchange
Enzyme Activation
Enzyme Stability
Escherichia coli
Mannans
Substrate Specificity
วันที่เผยแพร่: 2017
บทคัดย่อ: Endo-β-1,4-mannanases are important catalytic agents in several industries. The enzymes randomly cleave the β-1,4-linkage in the mannan backbone and release short β-1,4-mannooligosaccharides and mannose. In the present study, mannanase (ManS2) from thermotolerant Bacillus sp. SWU60 was purified, characterized, and its gene was cloned and overexpressed in Escherichia coli. ManS2 was purified from culture filtrate (300 ml) by using hydrophobic, ion-exchange, and size-exclusive liquid chromatography. The apparent molecular mass was 38 kDa. Optimal pH and temperature for enzyme activity were 6.0 and 60 °C, respectively. The enzyme was stable up to 60 °C for 1 h and at pH 5–9 at 4 °C for 16 h. Its enzyme activity was inhibited by Hg2+. The full-length mans2 gene was 1,008 bp, encoding a protein of 336 amino acids. Amino acid sequence analysis revealed that it belonged to glycoside hydrolase family 26. Konjac glucomannan was a favorable substrate for recombinant ManS2 (rManS2). rManS2 also degraded galactomannan from locust bean gum, indicating its potential for production of glucomanno- and galactomanno-oligosaccharides. Both native and recombinant ManS2 from Bacillus sp. SWU60 can be applied in several industries especially food and feed. © 2017, Springer Science+Business Media Dordrecht.
URI: https://ir.swu.ac.th/jspui/handle/123456789/13136
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85013287779&doi=10.1007%2fs11274-017-2224-7&partnerID=40&md5=d6213b37034c410a7b60720f4631acbe
ISSN: 9593993
Appears in Collections:Scopus 1983-2021

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