Please use this identifier to cite or link to this item: https://ir.swu.ac.th/jspui/handle/123456789/13109
ชื่อเรื่อง: Ethanolic extract of thevetia peruviana flowers enhances TNF-α and TRAIL-induced apoptosis of human cervical cancer cells via intrinsic and extrinsic pathways
ผู้แต่ง: Managit C.
Sakurai H.
Saiki I.
Keywords: alcohol
cardiac glycoside
caspase
death receptor
inhibitor of apoptosis protein
nicotinamide adenine dinucleotide adenosine diphosphate ribosyltransferase 1
plant extract
protein bcl xl
protein mcl 1
survivin
Thevetia peruviana extract
tumor necrosis factor
tumor necrosis factor related apoptosis inducing ligand
unclassified drug
X linked inhibitor of apoptosis
adenocarcinoma cell line
apoptosis
Article
B cell lymphoma
bark
cell proliferation
cell proliferation assay
cell viability
controlled study
down regulation
electrophoresis
flower
human
human cell
immunoblotting
stomach adenocarcinoma
uterine cervix cancer
Western blotting
วันที่เผยแพร่: 2017
บทคัดย่อ: Tumor necrosis factor-α (TNF-α) and TNF-related apoptosis-inducing ligand (TRAIL) are promising candidates for cancer treatment due to their ability to induce apoptosis through death receptor stimulation. However, their usage may be limited due to the resistance of cancer cells to TNF-α- and TRAIL-induced apoptosis. Currently, there is interest in screening for natural products that can sensitize cancer cells to TNF-α- and TRAIL-induced apoptosis for their use in combination with TNF-α or TRAIL. It was previously reported that the bark extract of Thevetia peruviana showed a reversal effect on TRAIL-resistance in human gastric adenocarcinoma cell lines. In the present study, the effects of the ethanolic extract of T. peruviana flowers on TNF-α- and TRAIL-induced apoptosis of human cervical cancer HeLa cells were investigated in vitro by determining cell viability and apoptosis using a WST-1 cell proliferation assay and immunoblot analysis, respectively. The ethanolic extract of T. peruviana flowers promoted TNF-α and TRAIL-mediated cell death through the activation of the caspase cascade, poly(ADP-ribose) polymerase and BH3-interacting domain death agonist cleavage. Combined treatment using the extract plus TNF-α resulted in downregulation of anti-apoptotic protein, including myeloid cell leukemia sequence-1, B-cell lymphoma-extra large (Bcl-XL), X-linked inhibitor of apoptosis protein and survivin, while the combined treatment with TRAIL downregulated Bcl-XL. Thus, the ethanolic extract of T. peruviana flowers has potential in sensitizing the TNF-α- and TRAIL-induced apoptosis of HeLa cells via the intrinsic and extrinsic pathways. © 2017, Spandidos Publications. All rights reserved.
URI: https://ir.swu.ac.th/jspui/handle/123456789/13109
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85015321143&doi=10.3892%2fol.2017.5748&partnerID=40&md5=af967ac93605f2a5832857b18a9e95e8
ISSN: 17921074
Appears in Collections:Scopus 1983-2021

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