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DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kaewphinit T. | |
dc.contributor.author | Ckumdee J. | |
dc.contributor.author | Chansiri K. | |
dc.contributor.author | Santiwatanakul S. | |
dc.date.accessioned | 2021-04-05T03:22:19Z | - |
dc.date.available | 2021-04-05T03:22:19Z | - |
dc.date.issued | 2017 | |
dc.identifier.issn | 2550857 | |
dc.identifier.other | 2-s2.0-85022222151 | |
dc.identifier.uri | https://ir.swu.ac.th/jspui/handle/123456789/13102 | - |
dc.identifier.uri | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85022222151&doi=10.4103%2fijmm.IJMM_15_333&partnerID=40&md5=f3db7e86384828e5b06f548d5e592dce | |
dc.description.abstract | Loop-mediated isothermal amplification (LAMP) has been proposed as an inexpensive and easy to perform assay for molecular diagnostics. We present a novel strategy for the detection of LAMP amplicons derived from Mycobacterium tuberculosis by the use of Au-nanoprobes. When applied to a total of 93 clinical specimens, the LAMP assay demonstrated sensitivity and specificity higher than that of polymerase chain reaction and culture. The Au-nanoprobe augmented LAMP test platform with its advantages of robust reagents and a simple colorimetric detection method can be adapted easily for the rapid detection of other infectious disease agents at a low cost. © 2017 Indian Journal of Medical Microbiology Published by Wolters Kluwer -Medknow. | |
dc.subject | Article | |
dc.subject | Au nanoprobe assay | |
dc.subject | bacterium detection | |
dc.subject | colorimetry | |
dc.subject | controlled study | |
dc.subject | diagnostic procedure | |
dc.subject | loop mediated isothermal amplification | |
dc.subject | microscopy | |
dc.subject | Mycobacterium tuberculosis | |
dc.subject | nonhuman | |
dc.subject | polymerase chain reaction | |
dc.subject | predictive value | |
dc.subject | sensitivity and specificity | |
dc.subject | sputum culture | |
dc.subject | sputum smear | |
dc.subject | tuberculosis | |
dc.subject | tuberculosis rapid test | |
dc.subject | cost | |
dc.subject | evaluation study | |
dc.subject | genetics | |
dc.subject | human | |
dc.subject | isolation and purification | |
dc.subject | molecular diagnosis | |
dc.subject | Mycobacterium tuberculosis | |
dc.subject | nanotechnology | |
dc.subject | nucleic acid amplification | |
dc.subject | procedures | |
dc.subject | time factor | |
dc.subject | tuberculosis | |
dc.subject | gold | |
dc.subject | metal nanoparticle | |
dc.subject | Colorimetry | |
dc.subject | Costs and Cost Analysis | |
dc.subject | Gold | |
dc.subject | Humans | |
dc.subject | Metal Nanoparticles | |
dc.subject | Molecular Diagnostic Techniques | |
dc.subject | Mycobacterium tuberculosis | |
dc.subject | Nanotechnology | |
dc.subject | Nucleic Acid Amplification Techniques | |
dc.subject | Sensitivity and Specificity | |
dc.subject | Time Factors | |
dc.subject | Tuberculosis | |
dc.title | Development and evaluation of a loop-mediated isothermal amplification combined with au-nanoprobe assay for rapid detection of Mycobacterium tuberculosis | |
dc.type | Article | |
dc.rights.holder | Scopus | |
dc.identifier.bibliograpycitation | Indian Journal of Medical Microbiology. Vol 35, No.2 (2017), p.302-304 | |
dc.identifier.doi | 10.4103/ijmm.IJMM_15_333 | |
Appears in Collections: | Scopus 1983-2021 |
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