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https://ir.swu.ac.th/jspui/handle/123456789/13102| Title: | Development and evaluation of a loop-mediated isothermal amplification combined with au-nanoprobe assay for rapid detection of Mycobacterium tuberculosis |
| Authors: | Kaewphinit T. Ckumdee J. Chansiri K. Santiwatanakul S. |
| Keywords: | Article Au nanoprobe assay bacterium detection colorimetry controlled study diagnostic procedure loop mediated isothermal amplification microscopy Mycobacterium tuberculosis nonhuman polymerase chain reaction predictive value sensitivity and specificity sputum culture sputum smear tuberculosis tuberculosis rapid test cost evaluation study genetics human isolation and purification molecular diagnosis Mycobacterium tuberculosis nanotechnology nucleic acid amplification procedures time factor tuberculosis gold metal nanoparticle Colorimetry Costs and Cost Analysis Gold Humans Metal Nanoparticles Molecular Diagnostic Techniques Mycobacterium tuberculosis Nanotechnology Nucleic Acid Amplification Techniques Sensitivity and Specificity Time Factors Tuberculosis |
| Issue Date: | 2017 |
| Abstract: | Loop-mediated isothermal amplification (LAMP) has been proposed as an inexpensive and easy to perform assay for molecular diagnostics. We present a novel strategy for the detection of LAMP amplicons derived from Mycobacterium tuberculosis by the use of Au-nanoprobes. When applied to a total of 93 clinical specimens, the LAMP assay demonstrated sensitivity and specificity higher than that of polymerase chain reaction and culture. The Au-nanoprobe augmented LAMP test platform with its advantages of robust reagents and a simple colorimetric detection method can be adapted easily for the rapid detection of other infectious disease agents at a low cost. © 2017 Indian Journal of Medical Microbiology Published by Wolters Kluwer -Medknow. |
| URI: | https://ir.swu.ac.th/jspui/handle/123456789/13102 https://www.scopus.com/inward/record.uri?eid=2-s2.0-85022222151&doi=10.4103%2fijmm.IJMM_15_333&partnerID=40&md5=f3db7e86384828e5b06f548d5e592dce |
| ISSN: | 2550857 |
| Appears in Collections: | Scopus 1983-2021 |
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